Shao Wenyong, Zhang Yu, Ren Weichao, Chen Changjun
College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Pestic Biochem Physiol. 2015 Jan;117:19-23. doi: 10.1016/j.pestbp.2014.10.003. Epub 2014 Oct 16.
Botrytis cinerea is a necrotrophic and filamentous fungus with a high risk of developing resistance to fungicides. The pyridinamine fungicide fluazinam has been reported to have excellent activity against B. cinerea and better effect on controlling gray mold. In this study, the physiological and biochemical characteristics of laboratory-induced mutants of B. cinerea with resistance to fluazinam has been investigated. Compared to the wild-type strains, the fluazinam-resistant mutants had a significant decrease in respiratory rate, glycerol, oxalate, and ATP contents, and an increase in ATPase activity and sensitivity to osmotic pressure, but did not differ in cell membrane permeability. Sequencing indicated that two parental strains and four resistant mutants were identical in the nucleotide sequence of F-ATPase gene. These results will enrich our understanding of the resistance mechanism of B. cinerea to fluazinam.
灰葡萄孢是一种具有高抗药性风险的坏死营养型丝状真菌。据报道，吡啶胺类杀菌剂氟唑菌酰胺对灰葡萄孢具有优异的活性，对防治灰霉病效果更佳。在本研究中，对实验室诱导产生的抗氟唑菌酰胺的灰葡萄孢突变体的生理生化特性进行了研究。与野生型菌株相比，抗氟唑菌酰胺突变体的呼吸速率、甘油、草酸盐和ATP含量显著降低，ATP酶活性和对渗透压的敏感性增加，但细胞膜通透性无差异。测序表明，两个亲本菌株和四个抗性突变体在F-ATP酶基因的核苷酸序列上是相同的。这些结果将丰富我们对灰葡萄孢对氟唑菌酰胺抗性机制的理解。
