Jiang Li, Yan Shuangshuang, Yang Wencai, Li Yanqiang, Xia Mengxue, Chen Zijing, Wang Qian, Yan Liying, Song Xiaofei, Liu Renyi, Zhang Xiaolan
Department of Vegetable Sciences, Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, China Agricultural University, Beijing 100193, China.
Shanghai Center for Plant Stress Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 201602, China.
Sci Rep. 2015 Jan 26;5:8031. doi: 10.1038/srep08031.
Cucumber (Cucumis sativus L.) fruit is a type of fleshy fruit that is harvested immaturely. Early fruit development directly determines the final fruit length and diameter, and consequently the fruit yield and quality. Different cucumber varieties display huge variations of fruit length, but how fruit length is determined at the molecular level remains poorly understood. To understand the genes and gene networks that regulate fruit length in cucumber, high throughout RNA-Seq data were used to compare the transcriptomes of early fruit from two near isogenic lines with different fruit lengths. 3955 genes were found to be differentially expressed, among which 2368 genes were significantly up-regulated and 1587 down-regulated in the line with long fruit. Microtubule and cell cycle related genes were dramatically activated in the long fruit, and transcription factors were implicated in the fruit length regulation in cucumber. Thus, our results built a foundation for dissecting the molecular mechanism of fruit length control in cucumber, a key agricultural trait of significant economic importance.
黄瓜（Cucumis sativus L.）果实是一种未成熟时就被采收的肉质果实。果实早期发育直接决定最终果实的长度和直径，进而决定果实产量和品质。不同黄瓜品种的果实长度差异巨大，但在分子水平上果实长度是如何决定的仍知之甚少。为了解调控黄瓜果实长度的基因和基因网络，利用高通量RNA-Seq数据比较了两个果实长度不同的近等基因系早期果实的转录组。发现3955个基因差异表达，其中2368个基因在长果系中显著上调，1587个基因下调。微管和细胞周期相关基因在长果中显著激活，转录因子参与黄瓜果实长度调控。因此，我们的结果为剖析黄瓜果实长度控制的分子机制奠定了基础，黄瓜果实长度是一个具有重要经济意义的关键农艺性状。
