Iverson S A, Brazil A M, Ferguson J M, Nelson K, Lautenbach E, Rankin S C, Morris D O, Davis M F
Department of Environmental Health Sciences, Johns Hopkins Bloomberg School of Public Health, 615 North Wolfe Street, Baltimore, MD 21205, USA.
Department of Environmental Health Sciences, Johns Hopkins Bloomberg School of Public Health, 615 North Wolfe Street, Baltimore, MD 21205, USA.
Vet Microbiol. 2015 Mar 23;176(1-2):202-8. doi: 10.1016/j.vetmic.2015.01.003. Epub 2015 Jan 12.
Methicillin-resistant strains of Staphylococcus aureus (MRSA), Staphylococcus pseudintermedius (MRSP), and other pathogenic staphylococci can cause infections in companion animals and humans. Identification of colonized animals is fundamental to research and practice needs, but harmonized methods have not yet been established. To establish the optimal anatomic site for the recovery of methicillin-resistant coagulase positive staphylococci (CPS), survey data and swabs were collected from 196 pets (dogs, cats, reptiles, birds, fish and pocket pets) that lived in households with an MRSA-infected person. Using broth-enrichment culture and PCR for speciation, S. aureus was identified in 27 of 179 (15%) pets sampled at baseline and 19 of 125 (15%) pets sampled at a three-month follow-up home visit. S. pseudintermedius was isolated from 33 of 179 (18%) pets sampled at baseline and 21 of 125 (17%) of pets sampled at follow-up. The baseline MRSA and MRSP prevalence was 8% and 1% respectively from 145 mammalian pets. The follow-up MRSA and MRSP prevalence was 7% and <1% respectively from 95 mammalian pets. The mouth was the most sensitive single site sampled for isolation of S. aureus and S. pseudintermedius in mammals. In a subset of pets, from which all available isolates were identified, dual carriage of S. aureus and S. pseudintermedius was 22% at baseline and 11% at follow-up. These results identify the mouth as the most sensitive site to screen for pathogenic staphylococci and suggest that it should be included in sampling protocols.
耐甲氧西林金黄色葡萄球菌（MRSA）、伪中间葡萄球菌（MRSP）及其他致病性葡萄球菌可在伴侣动物和人类中引发感染。确定定植动物对于研究和实践需求至关重要，但尚未建立统一的方法。为确定耐甲氧西林凝固酶阳性葡萄球菌（CPS）的最佳分离解剖部位，我们从196只生活在有MRSA感染患者家庭中的宠物（狗、猫、爬行动物、鸟类、鱼类和小型宠物）收集了调查数据和拭子样本。采用肉汤增菌培养和PCR进行菌种鉴定，在基线时采集的179只宠物中有27只（15%）鉴定出金黄色葡萄球菌，在三个月随访家庭访视时采集的125只宠物中有19只（15%）鉴定出金黄色葡萄球菌。在基线时采集的179只宠物中有33只（18%）分离出伪中间葡萄球菌，在随访时采集的125只宠物中有21只（17%）分离出伪中间葡萄球菌。在145只哺乳动物宠物中，基线时MRSA和MRSP的患病率分别为8%和1%。在95只哺乳动物宠物中，随访时MRSA和MRSP的患病率分别为7%和<1%。口腔是在哺乳动物中分离金黄色葡萄球菌和伪中间葡萄球菌最敏感的单一采样部位。在一个对所有可用分离株都进行了鉴定的宠物亚组中，金黄色葡萄球菌和伪中间葡萄球菌的双重携带率在基线时为22%，在随访时为11%。这些结果确定口腔是筛查致病性葡萄球菌最敏感的部位，并建议应将其纳入采样方案中。
