Figueiredo L T, Nogueira R M, Cavalcanti S M, Schatzmayr H, da Rosa A T
Departamento de Clínica Médica, Faculdade de Medicina de Ribeirão Preto, USP, Brasil.
Mem Inst Oswaldo Cruz. 1989 Jul-Sep;84(3):303-7. doi: 10.1590/s0074-02761989000300003.
This paper presents the evaluation of an enzyme immunoassay in which Mayaro virus-infected cultured cells are used as antigen (EIA-ICC) and an IgM antibody capture ELISA (MAC-ELISA) for Mayaro serologic diagnosis using 114 human sera obtained during a Mayaro outbreak occurred in Bolivia, in 1987. Results were compared with those obtained by haemagglutination-inhibition test (HAI). MAC-ELISA was the most sensitive technique for anti-Mayaro IgM detection. MAC-ELISA was twice as sensitive as IgM EIA-ICC. The data shows that MAC-ELISA is a practical and valid technique for diagnosis of recent Mayaro infection. IgG EIA-ICC showed high sensitivity and high specificity compared to HAI. The combination of anti-Mayaro IgG and IgM EIA-ICC results presented the highest sensitivity of the study. Anti-Mayaro IgG and IgM simultaneous detection by EIA-ICC can be used for recent infection diagnosis (in spite of a less sensitive IgM detection than by MAC-ELISA), for surveillance and sero-epidemiologic studies, and for studies of IgG and IgM responses to Mayaro infection.
本文介绍了一种酶免疫测定法的评估，其中使用马亚罗病毒感染的培养细胞作为抗原（酶免疫测定 - 感染细胞培养法，EIA - ICC），以及一种用于马亚罗血清学诊断的IgM抗体捕获酶联免疫吸附测定法（MAC - ELISA），该评估使用了1987年在玻利维亚马亚罗疫情期间获得的114份人类血清。将结果与血凝抑制试验（HAI）获得的结果进行了比较。MAC - ELISA是检测抗马亚罗IgM最敏感的技术。MAC - ELISA的敏感性是IgM EIA - ICC的两倍。数据表明，MAC - ELISA是诊断近期马亚罗感染的实用且有效的技术。与HAI相比，IgG EIA - ICC显示出高敏感性和高特异性。抗马亚罗IgG和IgM EIA - ICC结果的组合呈现出该研究的最高敏感性。通过EIA - ICC同时检测抗马亚罗IgG和IgM可用于近期感染诊断（尽管IgM检测的敏感性低于MAC - ELISA）、监测和血清流行病学研究，以及对马亚罗感染的IgG和IgM反应的研究。
