Carter I W, Smythe L D, Fraser J R, Stallman N D, Cloonan M J
Pathology. 1985 Jul;17(3):503-8. doi: 10.3109/00313028509105510.
An enzyme-linked immunosorbent assay based on antibody class capture was developed for the detection of Ross River virus-specific immunoglobulin M antibodies (RRV IgM). The assay was specific, reproducible and precise. When compared with conventional tests for the detection of RRV IgM, such as hemagglutination inhibition following sucrose density gradient centrifugation and indirect enzyme-linked immunosorbent assay, the class capture assay was more sensitive. In 186 sera which were collected from 39 patients with RRV infection over a period of 1-4 yr from onset of initial symptoms, RRV IgM persisted for at least 1-2 yr. Sera were tested both at a single dilution from which the results were expressed as a binding index and in a dilution series in which they were expressed as an antibody titre. Binding index values gave better discrimination between sera collected during acute and later phases of the disease and may be of greater value than antibody titres in the diagnosis of RRV infection.
开发了一种基于抗体类别捕获的酶联免疫吸附测定法，用于检测罗斯河病毒特异性免疫球蛋白M抗体（RRV IgM）。该测定法具有特异性、可重复性和精确性。与检测RRV IgM的传统试验（如蔗糖密度梯度离心后的血凝抑制试验和间接酶联免疫吸附测定法）相比，类别捕获测定法更灵敏。在从39例RRV感染患者中收集的186份血清中，这些血清是在初始症状出现后的1至4年期间采集的，RRV IgM持续至少1至2年。血清在单一稀释度下进行检测，结果以结合指数表示，同时也在稀释系列中进行检测，结果以抗体滴度表示。结合指数值在区分疾病急性期和后期采集的血清方面表现更好，在RRV感染的诊断中可能比抗体滴度更有价值。
