Ribeiro Emmeline Flor, de Fátima Reis Carolina, de Carvalho Flávio Silva, Abreu João Pedro Silva, Arruda Andréa Fernandes, Garrote Clévia Ferreira Duarte, Rocha Matheus Lavorenti
Faculty of Pharmacy, Federal University of Goias, Avenida Universitária s/n, 74605-220 Goiânia, GO, Brazil.
Institute of Chemistry, Federal University of Goias, Campus Samambaia, Caixa Postal 131, 74001-970 Goiânia, GO, Brazil.
J Ethnopharmacol. 2015 Apr 2;163:142-8. doi: 10.1016/j.jep.2015.01.023. Epub 2015 Jan 24.
Aspidosperma subincanum Mart. is a medicinal herb known for its diuretic properties and used for the treatment of cardiovascular-related illnesses. Although our earlier study has shown that the ethanol extract of Aspidosperma subincanum (EEAS) induces hypotension and vasodilation, no scientific data have been recorded to evaluate the diuretic effects of this Brazilian medicinal plant. The aim of this study was to evaluate the diuretic activity of EEAS, and possible mechanism of action, using Wistar rats.
EEAS (60 and 120mg/kg), furosemide (20mg/kg) or saline (control) were orally administered to rats individually held in metabolic cages for urine collection 1, 2, 4, 6, 8, 12 and 24h after treatment. In order to evaluate the involvement of prostaglandins in the diuretic action of EEAS, the animals received piroxicam (5mg/kgi.p.), a nonselective inhibitor of cyclooxygenase, before treatment with EEAS at 120mg/kg. The control groups received only saline (NaCl, 0.9%), or saline and piroxicam. Urinary volume, electrolyte excretion and pH were measured.
Oral administration of EEAS 60 and 120mg/kg significantly increased diuresis and electrolyte excretion of Na(+) and K(+) on a continuous basis throughout the study period. Both EEAS 60 and 120mg/kg caused a relative increase of around 77% and 142%, respectively, in cumulative diuresis compared with the control group. From 4th hour until the end of the experiment, the group treated with EEAS 120mg/kg provided a greater excretion of Na(+) than the furosemide group. The diuretic effects of EEAS were neutralized by piroxicam between 4 and 8h after treatment.
The results suggest that EEAS could present compound(s) responsible for diuretic activities, and the mechanism could involve the prostaglandin system.
亚渐尖叶白坚木（Aspidosperma subincanum Mart.）是一种药用植物，以其利尿特性而闻名，用于治疗心血管相关疾病。尽管我们早期的研究表明亚渐尖叶白坚木乙醇提取物（EEAS）可诱导低血压和血管舒张，但尚未有科学数据记录来评估这种巴西药用植物的利尿作用。本研究的目的是使用Wistar大鼠评估EEAS的利尿活性及其可能的作用机制。
将EEAS（60和120mg/kg）、呋塞米（20mg/kg）或生理盐水（对照组）分别口服给予单独饲养在代谢笼中的大鼠，在治疗后1、2、4、6、8、12和24小时收集尿液。为了评估前列腺素在EEAS利尿作用中的参与情况，在给予120mg/kg EEAS治疗前，动物接受吡罗昔康（5mg/kg，腹腔注射），一种环氧化酶的非选择性抑制剂。对照组仅接受生理盐水（0.9%氯化钠），或生理盐水和吡罗昔康。测量尿量、电解质排泄和pH值。
在整个研究期间，口服60和120mg/kg的EEAS持续显著增加尿量以及Na(+)和K(+)的电解质排泄。与对照组相比，60和120mg/kg的EEAS累积尿量分别相对增加了约77%和142%。从第4小时到实验结束，接受120mg/kg EEAS治疗的组比呋塞米组的Na(+)排泄量更大。治疗后4至8小时，吡罗昔康使EEAS的利尿作用消失。
结果表明EEAS可能含有具有利尿活性的化合物，其作用机制可能涉及前列腺素系统。
