McCafferty John, Schofield Darren
IONTAS Ltd., Babraham Research Campus, Cambridge CB22 3AT, UK.
MedImmune, Milstein Building, Granta Park, Cambridge CB21 6GH, UK.
Curr Opin Chem Biol. 2015 Jun;26:16-24. doi: 10.1016/j.cbpa.2015.01.003. Epub 2015 Jan 24.
The use of large genetically encoded binder libraries in co-operation with display technologies has matured over the past 25 years, and is now one of the primary methods used for selection of protein binders. Display technology has proven to be a robust and versatile method for generating binders to almost any antigen of interest. The evolution of this technology beyond antibody phage display has opened up new aspects for the concept of designer biologics. The ability to construct large populations of eukaryotic cells, including mammalian cells, where each cell expresses an individual antibody, peptide or engineered protein has added great value in identifying binders with desired properties. Here we review the evolution of display technology and highlight how it is being used today to generate binders with exquisite specificity, selectivity, affinity and developability characteristics.
在过去25年里，大型基因编码结合物文库与展示技术协同使用已渐趋成熟，如今它是用于筛选蛋白质结合物的主要方法之一。事实证明，展示技术是一种强大且通用的方法，可用于生成针对几乎任何感兴趣抗原的结合物。该技术从抗体噬菌体展示发展而来，为设计生物制剂的概念开辟了新的方向。构建大量真核细胞群体（包括哺乳动物细胞）的能力，其中每个细胞表达一种单独的抗体、肽或工程蛋白，这在鉴定具有所需特性的结合物方面具有巨大价值。在此，我们回顾展示技术的发展历程，并着重介绍其如今如何用于生成具有出色特异性、选择性、亲和力和可开发性特征的结合物。
