由树突状细胞引发的人类T淋巴细胞克隆性扩增。
Clonal expansion of human T lymphocytes initiated by dendritic cells.
作者信息
Langhoff E, Steinman R M
机构信息
Laboratory of Cellular Physiology and Immunology, Rockefeller University, New York, New York.
出版信息
J Exp Med. 1989 Jan 1;169(1):315-20. doi: 10.1084/jem.169.1.315.
Abstract
The accessory cell requirements for cloning T cells in the presence of lectin and T cell growth factors were examined with cells from human peripheral blood. We found that dendritic cells were active and perhaps essential. Single CD4+ lymphocytes could be cloned with 80% efficiency, and CD8+ cells with 50-60% efficiency if 10(3) syngeneic or allogeneic dendritic cells were added. Some T cell clones developed even with one dendritic cell. Monocytes or B lymphocytes from blood were at least 100-fold weaker in supporting clonal growth. These findings suggest a specialized feeder cell requirement, namely dendritic cells, for cloning T lymphocytes from single resting precursors.
摘要
在存在凝集素和T细胞生长因子的情况下，利用人外周血中的细胞研究了克隆T细胞对辅助细胞的需求。我们发现树突状细胞具有活性，甚至可能是必不可少的。如果添加10³个同基因或异基因树突状细胞，单个CD4⁺淋巴细胞的克隆效率可达80%，CD8⁺细胞的克隆效率为50 - 60%。甚至仅用一个树突状细胞就能产生一些T细胞克隆。血液中的单核细胞或B淋巴细胞在支持克隆生长方面的能力至少弱100倍。这些发现表明，从单个静止前体克隆T淋巴细胞需要一种特殊的饲养细胞，即树突状细胞。
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