Langhoff E, Kalland K H, Haseltine W A
Division of Human Retrovirology, Dana-Farber Cancer Institute, Boston, Massachusetts 02115.
J Clin Invest. 1993 Jun;91(6):2721-6. doi: 10.1172/JCI116512.
The rate and efficiency of key steps in the life cycle of the human immunodeficiency virus type 1 was examined in three primary cell types, T cells, monocytes, and T helper dendritic cells using the same quantity of virus involved and same cell number. The results show that viral DNA synthesis proceeds much more rapidly and efficiently in primary T helper dendritic cell populations than in primary T cell and monocyte populations. The increased rate of virus DNA synthesis is attributable either to an increase in the efficiency and the rate of uptake of the virus particles by the T helper dendritic cells, as compared with that in other cell types, or to an increased efficiency and rate of viral DNA synthesis in the T helper dendritic cells. In the subsequent phase of viral expression the appearance of spliced viral mRNA products also occur more rapidly in cultures of primary-blood-derived T helper dendritic cells than is the case in primary T cells and monocytes. The increased efficiency of the early steps of HIV-1 replication in primary-blood-derived T helper dendritic cells than in other blood-derived mononuclear cells raises the possibility that these cells play a central role in HIV-1 infection and pathogenesis.
使用相同数量的病毒和相同数量的细胞，在三种原代细胞类型（T细胞、单核细胞和T辅助树突状细胞）中检测了1型人类免疫缺陷病毒生命周期关键步骤的速率和效率。结果表明，与原代T细胞和单核细胞群体相比，病毒DNA合成在原代T辅助树突状细胞群体中进行得更快且更有效。病毒DNA合成速率的增加要么归因于与其他细胞类型相比，T辅助树突状细胞摄取病毒颗粒的效率和速率增加，要么归因于T辅助树突状细胞中病毒DNA合成的效率和速率增加。在病毒表达的后续阶段，原代血液来源的T辅助树突状细胞培养物中剪接的病毒mRNA产物的出现也比原代T细胞和单核细胞中的情况更快。1型人类免疫缺陷病毒在原代血液来源的T辅助树突状细胞中早期复制步骤的效率高于其他血液来源的单核细胞，这增加了这些细胞在HIV-1感染和发病机制中起核心作用的可能性。
