Kaushik Ajeet, Yndart Adriana, Jayant Rahul Dev, Sagar Vidya, Atluri Venkata, Bhansali Shekhar, Nair Madhavan
Center of Personalized Nanomedicine, Institute of Neuroimmune Pharmacology, Department of Immun ology, Herbert Wertheim College of Medicine, Florida International University, Miami, FL, USA.
BioMEMS Microsystems Laboratory, Department of Electrical and Computer Engineering, Florida International University, Miami, FL, USA.
Int J Nanomedicine. 2015 Jan 19;10:677-85. doi: 10.2147/IJN.S75514. eCollection 2015.
A novel electrochemical sensing method was devised for the first time to detect plasma cortisol, a potential psychological stress biomarker, in human immunodeficiency virus (HIV)-positive subjects. A miniaturized potentiostat (reconfigured LMP91000 chip) interfaced with a microfluidic manifold containing a cortisol immunosensor was employed to demonstrate electrochemical cortisol sensing. This fully integrated and optimized electrochemical sensing device exhibited a wide cortisol-detection range from 10 pg/mL to 500 ng/mL, a low detection limit of 10 pg/mL, and sensitivity of 5.8 μA (pg mL)(-1), with a regression coefficient of 0.995. This cortisol-selective sensing system was employed to estimate plasma cortisol in ten samples from HIV patients. The electrochemical cortisol-sensing performance was validated using an enzyme-linked immunosorbent assay technique. The results obtained using both methodologies were comparable within 2%-5% variation. The information related to psychological stress of HIV patients can be correlated with disease-progression parameters to optimize diagnosis, therapeutic, and personalized health monitoring.
首次设计了一种新型电化学传感方法，用于检测人类免疫缺陷病毒（HIV）阳性受试者血浆中的皮质醇，这是一种潜在的心理应激生物标志物。使用与包含皮质醇免疫传感器的微流体歧管相连的小型恒电位仪（重新配置的LMP91000芯片）来演示电化学皮质醇传感。这种完全集成且经过优化的电化学传感装置显示出10 pg/mL至500 ng/mL的宽皮质醇检测范围、10 pg/mL的低检测限以及5.8 μA（pg mL）（-1）的灵敏度，回归系数为0.995。该皮质醇选择性传感系统用于估计10例HIV患者样本中的血浆皮质醇。使用酶联免疫吸附测定技术验证了电化学皮质醇传感性能。两种方法获得的结果在2%-5%的变化范围内具有可比性。与HIV患者心理应激相关的信息可与疾病进展参数相关联，以优化诊断、治疗和个性化健康监测。
