Nagasawa Azumi, Sakasai Mitsuyoshi, Sakaguchi Daishi, Moriwaki Shigeru, Nishizawa Yoshinori, Kitahara Takashi
Nat Prod Commun. 2014 Dec;9(12):1733-6.
One new (1) and two known angular-type (2,3) furocoumarins, isoarchangelicin (1), archangelicin (2), and 2'-angeloyl-3'-isovaleryl vaginate (3), were isolated from the roots of Angelica atropurpurea. The structure of the new compound was established on the basis of one- and two-dimensional NMR spectra and other spectroscopic studies. The inhibitory activity of these three compounds and a deacylated form of archangelicin (4) on the nuclear factor of activated T cells (NFAT) signal transduction pathway was tested by NFAT-responsive luciferase reporter gene assay in cultured cells. Although 4 did not exhibit inhibitory activity on NFAT signaling, 1-3 exhibited dose-dependent inhibition with IC50 values of 16.5 (1), 9.0 (2), and 9.2 (3) μM.
从紫花前胡的根部分离出一种新的（1）和两种已知的角型（2,3）呋喃香豆素，异白芷内酯（1）、白芷内酯（2）和2'-当归酰基-3'-异戊酰基鞘蕊花内酯（3）。通过一维和二维核磁共振光谱以及其他光谱研究确定了新化合物的结构。在培养细胞中，通过NFAT反应性荧光素酶报告基因测定法测试了这三种化合物以及去酰基化形式的白芷内酯（4）对活化T细胞核因子（NFAT）信号转导途径的抑制活性。尽管4对NFAT信号没有抑制活性，但1-3表现出剂量依赖性抑制，IC50值分别为16.5（1）、9.0（2）和9.2（3）μM。
