Kofler R, Duchosal M A, Dixon F J
Department of Immunology, Research Institut of Scripps Clinic, La Jolla, CA 92037.
Immunogenetics. 1989;29(2):65-74. doi: 10.1007/BF00395853.
To define the polymorphism and extent of the mouse immunoglobulin kappa (Igk) gene complex, we have analyzed restriction-enzyme digested genomic DNA from 33 inbred strains of mice with labeled DNA probes corresponding to 16 Vk protein groups (1 of them previously undescribed) and the Jk/Ck region (V, variable; J, joining; C, constant). These probes detected between 1 and 25 distinct restriction enzyme fragments (REF) that appeared in up to eight polymorphic patterns, thus defining eight mouse Igk haplotypes. The investigated portion of the Vk repertoire was estimated to encompass between 60 and 120 discernable Vk gene-containing REFs. In contrast to mouse VH gene families, several Vk gene families defined by these probes appeared to overlap. This observation has implications for Vk gene analyses by nucleic acid hybridization and raises the possibility that the Vk gene complex is a continuum of related sequences.
为了定义小鼠免疫球蛋白κ（Igk）基因复合体的多态性和范围，我们用与16个Vk蛋白组（其中1个先前未描述）以及Jk/Ck区域（V，可变区；J，连接区；C，恒定区）对应的标记DNA探针，分析了来自33个近交系小鼠的经限制性内切酶消化的基因组DNA。这些探针检测到1至25个不同的限制性酶切片段（REF），它们呈现出多达八种多态性模式，从而定义了八种小鼠Igk单倍型。据估计，所研究的Vk基因库部分包含60至120个可分辨的含Vk基因的REF。与小鼠VH基因家族不同，由这些探针定义的几个Vk基因家族似乎有重叠。这一观察结果对通过核酸杂交进行Vk基因分析有影响，并增加了Vk基因复合体是相关序列连续体的可能性。
