Rattanavichai Wutti, Cheng Winton
Department of Tropical Agriculture and International Cooperation, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan, ROC.
Department of Aquaculture, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan, ROC.
Fish Shellfish Immunol. 2015 Apr;43(2):415-26. doi: 10.1016/j.fsi.2015.01.011. Epub 2015 Jan 26.
In the present study, Macrobrachium rosenbergii were fed with diets containing extracts of banana, Musa acuminate, fruit's peel (banana peels extract, BPE) at 0, 1.0, 3.0 and 6.0 g kg(-1). The non-specific immune parameters, disease resistance and anti-hypothermal stress were evaluated at 2, 4, 8, 16 and 32 days of post feeding. Also, we demonstrated the percent weight gain (PWG), percent length gain (PLG), feeding efficiency (FE), and survival rate of giant freshwater prawn at 30, 60, 90, and 120 days of post feeding. The PWG, PLG, FE and survival rate of prawns fed at 0, 1.0, 3.0 and 6.0 g kg(-1) BPE-containing diets after 120 days were 69.5%, 75.4%, 77.8% and 83.3%; 21.8%, 23.6%, 27.8% and 33.9%; 0.60, 0.72, 0.75 and 0.90; and 55.4%, 62.2%, 62.3% and 75.3%, respectively. After 32 days of post feeding, a significant increase in total haemocyte count (THC), different haemocyte count (DHC), respiratory bursts (RBs), superoxide dismutase (SOD) activity, glutathione peroxidase (GPx) activity, phenoloxidase (PO) activity and transglutaminase (TG) activity, and meanwhile, a decreased haemolymph coagulation time was observed. Furthermore, phagocytic activity and clearance efficiency of prawns against Lactococcus garvieae infection were significantly increased. Prawns challenged with L. garvieae after 32 days of feeding at 1.0, 3.0 and 6.0 g kg(-1) had a significantly higher survival rate (33.3%, 40.0% and 56.7%) than those fed with the control diet. Subsequently, hypothermal (14 °C) stress was 43.4%, 50.0% and 50.0%, respectively. Altogether, we therefore recommend the dietary BPE administration at 6.0 g kg(-1) promotes growth, anti-hypothermal stress, and enhance immunity and resistance against L. garvieae in M. rosenbergii.
在本研究中，罗氏沼虾被投喂含有香蕉（尖叶蕉）果皮提取物（香蕉皮提取物，BPE）的饲料，添加量分别为0、1.0、3.0和6.0 g kg⁻¹。在投喂后的第2、4、8、16和32天评估非特异性免疫参数、抗病能力和抗低温应激能力。此外，我们还测定了投喂后30、60、90和120天巨型淡水虾的体重增加百分比（PWG）、体长增加百分比（PLG）、摄食效率（FE）和存活率。投喂含1.0、3.0和6.0 g kg⁻¹ BPE饲料120天后，虾的PWG、PLG、FE和存活率分别为69.5%、75.4%、77.8%和83.3%；21.8%、23.6%、27.8%和33.9%；0.60、0.72、0.75和0.90；以及55.4%、62.2%、62.3%和75.3%。投喂32天后，总血细胞计数（THC）、不同血细胞计数（DHC）、呼吸爆发（RB）、超氧化物歧化酶（SOD）活性、谷胱甘肽过氧化物酶（GPx）活性、酚氧化酶（PO）活性和转谷氨酰胺酶（TG）活性显著增加，同时血淋巴凝固时间缩短。此外，虾对加氏乳杆菌感染的吞噬活性和清除效率显著提高。在1.0、3.0和6.0 g kg⁻¹剂量下投喂32天后用加氏乳杆菌攻毒的虾，其存活率（33.3%、40.0%和56.7%）显著高于投喂对照饲料的虾。随后，在14℃低温应激下的存活率分别为43.4%、50.0%和50.0%。因此，我们总体建议以6.0 g kg⁻¹的剂量在饲料中添加BPE可促进罗氏沼虾生长、提高抗低温应激能力，并增强其免疫力和对加氏乳杆菌的抵抗力。
