Yang Yujie, Sun Jia, Tang Kai, Lin Dan, Li Chenlan, Lin Yingfang
State Key Laboratory for Marine Environmental Science, Institute of Marine Microbes and Ecospheres, Xiamen University, Xiamen 361005, PR China.
Int J Syst Evol Microbiol. 2015 Apr;65(Pt 4):1247-1250. doi: 10.1099/ijs.0.000086. Epub 2015 Jan 29.
A Gram-stain-negative, rod-shaped, non-motile, aerobic bacterium, strain JL351(T), was isolated from the surface seawater of the South China Sea. Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain had a close relationship with members of the genera Ponticoccus , Antarctobacter and Sagittula , and the closest relative was Ponticoccus litoralis CL-GR66(T) (with 96.56% 16S rRNA gene sequence similarity). The polar lipids of strain JL351(T) comprised diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, three unidentified aminolipids, three unidentified phospholipids and an unidentified glycolipid. The predominant isoprenoid quinone was Q-10. The major fatty acids were C(18 : 1)ω7c (60.9%), C(18 : 0) (13.7%), C(16 : 0) (9.4%), 11-methyl C(18 : 1)ω7c (4.5%), and C(12 : 1) 3-OH (4.4%). The DNA G+C content was 66.2 mol%. Based on phenotypic, phylogenetic and genotypic data, strain JL351(T) is considered to represent a novel species in the genus Ponticoccus , for which the name Ponticoccus lacteus sp. nov. is proposed. The type strain is JL351(T) ( = CGMCC 1.12986(T) = JCM 30379(T)).
从中国南海表层海水中分离出一株革兰氏染色阴性、杆状、无运动性、需氧的细菌，菌株JL351(T)。基于16S rRNA基因序列的系统发育分析表明，该菌株与Ponticoccus属、Antarctobacter属和Sagittula属的成员关系密切，最接近的亲缘种是海滨Ponticoccus litoralis CL-GR66(T)（16S rRNA基因序列相似性为96.56%）。菌株JL351(T)的极性脂类包括双磷脂酰甘油、磷脂酰胆碱、磷脂酰甘油、磷脂酰乙醇胺、三种未鉴定的氨基脂类、三种未鉴定的磷脂类和一种未鉴定的糖脂类。主要的类异戊二烯醌为Q-10。主要脂肪酸为C(18 : 1)ω7c（60.9%）、C(18 : 0)（13.7%）、C(16 : 0)（9.4%）、11-甲基C(18 : 1)ω7c（4.5%）和C(12 : 1) 3-OH（4.4%）。DNA G+C含量为66.2 mol%。基于表型、系统发育和基因型数据，菌株JL351(T)被认为代表Ponticoccus属中的一个新物种，为此提出Ponticoccus lacteus sp. nov.这个名称。模式菌株为JL351(T)（=CGMCC 1.12986(T)=JCM 30379(T)）。
