Department of Chemical Engineering, Pohang University of Science and Technology, San 31, Hyoja-Dong, Pohang 790-784, Republic of Korea.
Gyeongbuk Sea Grant Institute, Pohang University of Science and Technology, San 31, Hyoja-Dong, Pohang 790-784, Republic of Korea.
Int J Syst Evol Microbiol. 2013 Jun;63(Pt 6):2101-2107. doi: 10.1099/ijs.0.040766-0. Epub 2012 Oct 19.
A novel bacterium, designated strain F028-2(T), was isolated from seawater at Damupo beach in Pohang, Korea, and investigated in a taxonomic study using a polyphasic approach. This novel strain was strictly aerobic, non-motile, Gram-stain-negative and rod-shaped, and occasionally formed aggregates. The temperature, pH and NaCl ranges for growth were 4-30 °C, pH 6.5-9.0 and 1-7% (w/v), respectively. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain F028-2(T) formed a lineage within the family Rhodobacteraceae of the class Alphaproteobacteria, and was closely related to members of the genera Sagittula and Antarctobacter with 96.3-96.4% sequence similarities. The polar lipid profile of strain F028-2(T) comprised diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminolipids, one unidentified phospholipid and six unidentified lipids. The predominant cellular fatty acids were C18:1ω7c and C12:1 3-OH. The genomic DNA G+C content of strain F028-2(T) was 61.6 mol% and the major respiratory quinone was Q-10. Based on phenotypic, phylogenetic and genotypic data, strain F028-2(T) is considered to represent a novel species in the genus Sagittula, for which the name Sagittula marina sp. nov. is proposed. The type strain is F028-2(T) (=KCTC 23543(T)=JCM 17627(T)). An emended description of the genus Sagittula is also proposed.
一株新型细菌，命名为 F028-2(T) 株，从韩国浦项市 Damupo 海滩的海水中分离得到，并用多相分类法进行了分类学研究。该新菌株是严格需氧的、非运动的、革兰氏阴性的杆状的，偶尔形成聚集体。生长的温度、pH 和 NaCl 范围分别为 4-30°C、pH 6.5-9.0 和 1-7%（w/v）。基于 16S rRNA 基因序列的系统发育分析表明，F028-2(T) 株在α变形菌纲的红杆菌科内形成一个分支，与 Sagittula 和 Antarctobacter 属的成员密切相关，序列相似度为 96.3-96.4%。F028-2(T) 株的极性脂谱由二磷脂酰甘油、磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰甘油、两种未鉴定的胺脂、一种未鉴定的磷脂和六种未鉴定的脂组成。主要的细胞脂肪酸为 C18:1ω7c 和 C12:1 3-OH。F028-2(T) 株的基因组 DNA G+C 含量为 61.6mol%，主要的呼吸醌为 Q-10。基于表型、系统发育和基因型数据，F028-2(T) 株被认为代表 Sagittula 属的一个新型种，提议命名为海洋 Sagittula 新种。模式菌株为 F028-2(T)（=KCTC 23543(T)=JCM 17627(T)）。还提出了 Sagittula 属的修订描述。
