Zhang Guishan, Haroon Mohamed Fauzi, Zhang Ruifu, Dong Xiaoyan, Liu Di, Xiong Qin, Xun Weibing, Dong Xiuzhu, Stingl Ulrich
King Abdullah University of Science and Technology (KAUST), Red Sea Research Center, Thuwal 23955-6900, Saudi Arabia.
Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Key Laboratory of Microbial Resources Collection and Preservation, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
Int J Syst Evol Microbiol. 2017 Nov;67(11):4358-4364. doi: 10.1099/ijsem.0.002280. Epub 2017 Oct 6.
Strain SJ5A-1, a Gram-stain-negative, coccus-shaped, non-motile, aerobic bacterium, was isolated from the brine-seawater interface of the Erba Deep in the Red Sea, Saudi Arabia. The colonies of strain SJ5A-1 have a beige to pale-brown pigmentation, are approximately 0.5-0.7 µm in diameter, and are catalase and oxidase positive. Growth occurred optimally at 30-33 °C, pH 7.0-7.5, and in the presence of 9.0-12.0 % NaCl (w/v). Phylogenetic analysis of the 16S rRNA gene indicates that strain SJ5A-1 is a member of the genus Ponticoccus within the family Rhodobacteraceae. Ponticoccus litoralis DSM 18986 is the most closely related described species based on 16S rRNA gene sequence identity (96.7 %). The DNA-DNA hybridization value between strain SJ5A-1 and P. litoralis DSM 18986 was 36.7 %. The major respiratory quinone of strain SJ5A-1 is Q-10; it predominantly uses the fatty acids C18 : 1 (54.2 %), C18 : 0 (11.2 %), C16 : 0 (8.6 %), 11-methyl C18 : 1ω7c (7.7 %), C19 : 0cyclo ω8c (3.3 %), and C12 : 1 3-OH (3.5 %), and its major polar lipids are phosphatidylethanolamine, phosphatidylglycerol, phosphocholine, an unknown aminolipid, an unknown phospholipid and two unknown lipids. The genome draft of strain SJ5A-1 as presented here is 4 562 830 bp in size and the DNA G+C content is 68.0 mol%. Based on phenotypic, phylogenetic and genotypic data, strain SJ5A-1 represents a novel species in the genus Ponticoccus, for which we propose the name Ponticoccus marisrubri sp. nov. The type strain of P. marisrubri is SJ5A-1 (=JCM 19520=ACCC19863).
菌株SJ5A-1是一种革兰氏阴性、球状、无运动性的需氧细菌，从沙特阿拉伯红海埃尔巴深海的卤水-海水界面分离得到。菌株SJ5A-1的菌落呈米色至浅棕色，直径约为0.5-0.7 µm，过氧化氢酶和氧化酶呈阳性。在30-33 °C、pH 7.0-7.5以及9.0-12.0%（w/v）NaCl存在的条件下生长最佳。对16S rRNA基因的系统发育分析表明，菌株SJ5A-1是红杆菌科Ponticoccus属的成员。基于16S rRNA基因序列同一性（96.7%），Ponticoccus litoralis DSM 18986是与其关系最密切的已描述物种。菌株SJ5A-1与Ponticoccus litoralis DSM 18986之间的DNA-DNA杂交值为36.7%。菌株SJ5A-1的主要呼吸醌为Q-10；它主要利用脂肪酸C18 : 1（54.2%）、C18 : 0（11.2%）、C16 : 0（8.6%）、11-甲基C18 : 1ω7c（7.7%）、C19 : 0环ω8c（3.3%）和C12 : 1 3-OH（3.5%），其主要极性脂类为磷脂酰乙醇胺、磷脂酰甘油、磷酸胆碱、一种未知氨基脂、一种未知磷脂和两种未知脂类。此处呈现的菌株SJ5A-1的基因组草图大小为4 562 830 bp，DNA G+C含量为68.0 mol%。基于表型、系统发育和基因型数据，菌株SJ5A-1代表Ponticoccus属中的一个新物种，我们将其命名为Ponticoccus marisrubri sp. nov.。Ponticoccus marisrubri的模式菌株为SJ5A-1（=JCM 19520=ACCC19863）。
