Zhang Lan, Han Yong, Tan Guoxin
State-key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an 710049, China.
State-key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an 710049, China.
Colloids Surf B Biointerfaces. 2015 Mar 1;127:8-14. doi: 10.1016/j.colsurfb.2015.01.017. Epub 2015 Jan 17.
Percutaneous implant requires a tight bond between the underlying dermis of skin and implant surface to prevent epithelial down-growth and infection, while fibroblasts play a key role in the skin-implant integration. In this work, nanorod-shaped hydroxyaptite (HA) with a mean diameter of 70 nm and length of 400 nm was hydrothermally grown on micro-arc oxidized (MAOed) Ca- and P-doped ZrO2 to form a bilayer coating. The hydrothermal formation mechanism of HA nanorods was explored, and the adsorption of total protein on the coating from α-MEM medium containing 10% fetal bovine serum was examined. Employing L-929 cells, the behaviors of fibroblasts on the bilayer coating, including adhesion and proliferation were evaluated together the polished Zr and as-MAOed ZrO2. The obtained results show that the HA nanorods nucleated on ZrO2 and grew at the expense of the doped Ca and P ions during the hydrothermal treatment (HT). The HA nanorods patterned coating enhanced protein absorption, and significantly improved the adhesion and proliferation of fibroblasts compared to the as-MAOed ZrO2 and polished Zr. It suggests that the HA nanorods/ZrO2 coated zirconium has a potential application for percutaneous implants to enhance the attachment of skin.
经皮植入物需要皮肤下层真皮与植入物表面紧密结合，以防止上皮细胞向内生长和感染，而成纤维细胞在皮肤与植入物的整合中起关键作用。在本研究中，平均直径为70 nm、长度为400 nm的纳米棒状羟基磷灰石（HA）通过水热法生长在微弧氧化（MAOed）的钙磷掺杂ZrO2上，形成双层涂层。探讨了HA纳米棒的水热形成机制，并检测了其在含10%胎牛血清的α-MEM培养基中对涂层上总蛋白的吸附。使用L-929细胞，评估了成纤维细胞在双层涂层上的行为，包括粘附和增殖，并与抛光Zr和MAOed ZrO2进行了比较。结果表明，在水热处理（HT）过程中，HA纳米棒在ZrO2上形核并以掺杂的Ca和P离子为代价生长。与MAOed ZrO2和抛光Zr相比，HA纳米棒图案化涂层增强了蛋白质吸收，并显著改善了成纤维细胞的粘附和增殖。这表明HA纳米棒/ZrO2涂层锆在经皮植入物中具有增强皮肤附着的潜在应用。
