Taysi S, Keles M S, Gumustekin K, Akyuz M, Boyuk A, Cikman O, Bakan N
Department of Medical Biochemistry, Gaziantep University, School of Medicine, Gaziantep, Turkey.
Eur Rev Med Pharmacol Sci. 2015 Jan;19(1):154-60.
The aim of this study was to evaluate plasma homocysteine (Hcy), malondialdehyde (MDA), glutathione (GSH) levels, glutathione peroxidase (GSH-Px) and glutathione-S-transferase (GST) activities and liver tissue S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) levels in control and vitamin B6-deficient rats.
Thirty-two male rats with a weight of 65-75 g were used for the experiment. The rats were divided into control (n=16) and vitamin B6-deficient groups. At the end of the experiment, the animals were anesthetized with ketamine-HCl (Ketalar, 20 mg/kg, i.p.), and the blood was collected by cardiac puncture after thoracotomy. Plasma Hcy, pyridoxal phosphate (PLP), liver SAM, SAH levels measured by an isocratic system with high performance liquid chromatography. Plasma GSH-Px, GSH activities and GSH, MDA levels were carried out using a spectrophotometer.
Plasma Hcy, MDA, liver tissue SAH levels were significantly increased, whereas plasma GSH, PLP, liver tissue SAM levels, plasma GST, GSH-Px activities and SAM/SAH ratio were decreased compared to those of control group.
Vitamin B6 deficiency causes an increase in plasma homocysteine levels. Thus, we think that vitamin B6 supplementation could be used for therapeutic purposes in hyperhomocysteinemia condition.
本研究旨在评估对照大鼠和维生素B6缺乏大鼠的血浆同型半胱氨酸（Hcy）、丙二醛（MDA）、谷胱甘肽（GSH）水平、谷胱甘肽过氧化物酶（GSH-Px）和谷胱甘肽-S-转移酶（GST）活性以及肝组织S-腺苷甲硫氨酸（SAM）和S-腺苷同型半胱氨酸（SAH）水平。
选用32只体重65 - 75 g的雄性大鼠进行实验。将大鼠分为对照组（n = 16）和维生素B6缺乏组。实验结束时，用氯胺酮-HCl（凯他敏，20 mg/kg，腹腔注射）麻醉动物，开胸后经心脏穿刺采血。采用高效液相色谱等度系统测定血浆Hcy、磷酸吡哆醛（PLP）、肝组织SAM、SAH水平。用分光光度计检测血浆GSH-Px、GSH活性以及GSH、MDA水平。
与对照组相比，血浆Hcy、MDA、肝组织SAH水平显著升高，而血浆GSH、PLP、肝组织SAM水平、血浆GST、GSH-Px活性以及SAM/SAH比值降低。
维生素B6缺乏导致血浆同型半胱氨酸水平升高。因此，我们认为补充维生素B6可用于高同型半胱氨酸血症的治疗。
