Wang Li-Juan, Hu Ya-Qiong, Yin Shou-Wei, Yang Xiao-Quan, Lai Fu-Rao, Wang Si-Qi
Research and Development Center of Food Proteins, Department of Food Science and Technology, South China University of Technology , Guangzhou 510640, People's Republic of China.
J Agric Food Chem. 2015 Mar 11;63(9):2514-24. doi: 10.1021/jf505227a. Epub 2015 Feb 25.
Lipid peroxidation in oil-in-water (o/w) emulsions leads to rancidity and carcinogen formation. This work attempted to protect lipid droplets of emulsions from peroxidation via manipulation of the emulsions' interface framework using dual-function zein/CH complex particles (ZCPs). ZCP with intermediate wettability was fabricated via a simple antisolvent approach. Pickering emulsions were produced via a simple and inexpensive shear-induced emulsification technique. ZCP was irreversibly anchored at the oil-water interface to form particle-based network architecture therein, producing ultrastable o/w Pickering emulsions (ZCPEs). ZCPE was not labile to lipid oxidation, evidenced by low lipid hydroperoxides and malondialdehyde levels in the emulsions after thermally accelerated storage. The targeted accumulation of curcumin, a model antioxidant, at the interface was achieved using the ZCP as interfacial vehicle, forming antioxidant shells around dispersed droplets. The oxidative stability of ZCPEs was further improved. Interestingly, no detectable hexanal peak appeared in headspace gas chromatography of the Pickering emulsions. The novel interfacial architecture via the combination of steric hindrance from ZCP-based membrane and interfacial cargo of curcumin endowed the emulsions with favorable oxidative stability. This study opens a promising pathway for producing antioxidant emulsions via the combination of Pickering stabilization mechanism and interfacial delivery of antioxidant.
水包油（o/w）乳液中的脂质过氧化会导致酸败和致癌物形成。这项工作试图通过使用双功能玉米醇溶蛋白/壳聚糖复合物颗粒（ZCPs）来操纵乳液的界面框架，从而保护乳液中的脂质滴免受过氧化。通过一种简单的反溶剂方法制备了具有中等润湿性的ZCP。通过一种简单且廉价的剪切诱导乳化技术制备了Pickering乳液。ZCP不可逆地锚定在油水界面，在其中形成基于颗粒的网络结构，从而产生超稳定的o/w Pickering乳液（ZCPEs）。热加速储存后乳液中脂质氢过氧化物和丙二醛水平较低，证明ZCPE对脂质氧化不敏感。使用ZCP作为界面载体，使姜黄素（一种模型抗氧化剂）在界面处实现靶向积累，在分散的液滴周围形成抗氧化壳。ZCPEs的氧化稳定性进一步提高。有趣的是，在Pickering乳液的顶空气相色谱中未出现可检测到的己醛峰。基于ZCP的膜的空间位阻与姜黄素的界面负载相结合形成的新型界面结构，赋予了乳液良好的氧化稳定性。这项研究为通过Pickering稳定机制与抗氧化剂的界面递送相结合来生产抗氧化乳液开辟了一条有前景的途径。
