Dodd Rebecca D, Añó Leonor, Blum Jordan M, Li Zhizhong, Van Mater David, Kirsch David G
Duke University Medical Center, Box 91006, Durham, NC, 27708, USA.
Methods Mol Biol. 2015;1267:283-95. doi: 10.1007/978-1-4939-2297-0_13.
We discuss the generation of primary soft tissue sarcomas in mice using the Cre-loxP system to activate conditional mutations in oncogenic Kras and the tumor suppressor p53 (LSL-Kras(G12D/+); p53(flox/flox)). Sarcomas can be generated either by adenoviral delivery of Cre recombinase, activation of transgenic Cre recombinase with tamoxifen, or through transplantation of isolated satellite cells with Cre activation in vitro. Various applications of these models are discussed, including anticancer therapies, metastasis, in vivo imaging, and genetic requirements for tumorigenesis.
我们讨论了利用Cre-loxP系统在小鼠中产生原发性软组织肉瘤的方法，该系统用于激活致癌性Kras和肿瘤抑制因子p53中的条件性突变（LSL-Kras(G12D/+); p53(flox/flox)）。肉瘤可以通过腺病毒递送Cre重组酶、用他莫昔芬激活转基因Cre重组酶，或者通过移植在体外激活了Cre的分离卫星细胞来产生。文中讨论了这些模型的各种应用，包括抗癌治疗、转移、体内成像以及肿瘤发生的遗传需求。
