Hauser Paul J, VanGordon Samuel B, Seavey Jonathan, Sofinowski Troy M, Ramadan Mohammad, Abdullah Shivon, Buffington C A Tony, Hurst Robert E
Department of Urology, College of Medicine, Oklahoma University Health Sciences Center, Oklahoma City, Oklahoma.
Department of Urology, College of Medicine, Oklahoma University Health Sciences Center, Oklahoma City, Oklahoma; General Surgery Department, Walter Reed National Military Medical Center, Bethesda, Maryland.
J Urol. 2015 Aug;194(2):571-7. doi: 10.1016/j.juro.2015.01.090. Epub 2015 Jan 28.
We analyzed the urothelium of cats diagnosed with feline interstitial cystitis to determine whether abnormalities in protein expression patterns could be detected and whether the expression pattern was similar to that in patients with human interstitial cystitis/bladder pain syndrome. The proteins analyzed are involved in cell adhesion and barrier function, comprise the glycosaminoglycan layer or are differentiation markers.
Formalin fixed biopsies from 8 cats with feline interstitial cystitis and from 7 healthy control cats were labeled by immunohistochemistry and scored with a modified version of a system previously used for human samples. Cluster analysis was performed to investigate relationships between markers and samples.
Of the feline interstitial cystitis bladders 89% showed abnormal protein expression and chondroitin sulfate patterns while only 27% of normal tissues showed slight abnormalities. Abnormalities were found in most feline interstitial cystitis samples, including biglycan in 87.5%, chondroitin sulfate, decorin, E-cadherin and keratin-20 in 100%, uroplakin in 50% and ZO-1 in 87.5%. In feline interstitial cystitis bladders about 75% of chondroitin sulfate, biglycan and decorin samples demonstrated absent luminal staining or no staining. Cluster analysis revealed that feline interstitial cystitis and normal samples could be clearly separated into 2 groups, showing that the urothelium of cats with feline interstitial cystitis is altered from normal urothelium.
Feline interstitial cystitis produces changes in luminal glycosaminoglycan and several proteins similar to that in patients, suggesting some commonality in mechanism. Results support the use of feline interstitial cystitis as a model of human interstitial cystitis.
我们分析了被诊断为猫间质性膀胱炎的猫的尿路上皮，以确定是否能检测到蛋白质表达模式的异常，以及其表达模式是否与人类间质性膀胱炎/膀胱疼痛综合征患者相似。所分析的蛋白质参与细胞黏附与屏障功能、构成糖胺聚糖层或为分化标志物。
对8只患有猫间质性膀胱炎的猫和7只健康对照猫的福尔马林固定活检组织进行免疫组织化学标记，并用先前用于人类样本的系统的改良版进行评分。进行聚类分析以研究标志物与样本之间的关系。
89%的猫间质性膀胱炎膀胱显示出异常的蛋白质表达和硫酸软骨素模式，而正常组织中只有27%显示出轻微异常。在大多数猫间质性膀胱炎样本中发现了异常，包括双糖链蛋白聚糖在87.5%的样本中异常，硫酸软骨素、核心蛋白聚糖、E-钙黏蛋白和角蛋白-20在100%的样本中异常，尿血小板溶素在50%的样本中异常，紧密连接蛋白-1在87.5%的样本中异常。在猫间质性膀胱炎膀胱中，约75%的硫酸软骨素、双糖链蛋白聚糖和核心蛋白聚糖样本显示管腔染色缺失或无染色。聚类分析显示，猫间质性膀胱炎样本和正常样本可清晰分为两组，表明患有猫间质性膀胱炎的猫的尿路上皮与正常尿路上皮有所不同。
猫间质性膀胱炎会导致管腔糖胺聚糖和几种蛋白质发生变化，与人类患者相似，提示在发病机制上存在一些共性。研究结果支持将猫间质性膀胱炎作为人类间质性膀胱炎的模型。
