Yamashita A, Hayashi M, Shimizu K, Oshima K
Department of Physiology, Kyoto University, Aichi, Japan.
Brain Res Dev Brain Res. 1989 Jan 1;45(1):103-11. doi: 10.1016/0165-3806(89)90012-6.
Distribution of somatostatin-immunoreactive cells in the cerebral cortex of macaque monkeys at embryonic day 120 (E120), E140, newborn, postnatal day 60 (P60) and adult stages were studied by the avidin-biotin-peroxidase immunohistochemical method. At all stages, there existed 3 types of cells in the gray matter: bipolar, multipolar and small-sized cells which stained only in perikaryon. Somatostatin-immunoreactive cells were observed from E120. The cell number increased between E120 and E140 and decreased until P60. At the newborn stage, a high density of cells was distributed in layer II of the prefrontal and parietal cortices (areas FD and PE). In layer I of the postcentral, parietal, temporal and preoccipital cortices (areas FA, PC, PE, TA, TE and OA), small numbers of horizontal cells were detected only at the embryonic and newborn stages. In adulthood, the number of somatostatin cells was much smaller than at the early stages (E140 and newborn). Compared to other cortical areas, in occipital cortex (area OC), there was little change in cell number during development. In occipito-temporal cortices, there were increases in cell number from posterior to anterior portion at all the stages. The large number of somatostatin cells in all layers of the cerebral cortex during the early stages indicates that somatostatin plays a role in the development of the monkey cerebral cortex.
采用抗生物素蛋白-生物素-过氧化物酶免疫组织化学方法，研究了猕猴胚胎第120天（E120）、E140、新生期、生后第60天（P60）和成年期大脑皮质中生长抑素免疫反应性细胞的分布。在所有阶段，灰质中均存在3种类型的细胞：双极细胞、多极细胞和仅胞体染色的小型细胞。在E120时观察到生长抑素免疫反应性细胞。细胞数量在E120至E140之间增加，直至P60时减少。在新生期，高密度的细胞分布在前额叶和顶叶皮质的II层（FD区和PE区）。在中央后回、顶叶、颞叶和枕前叶皮质的I层（FA区、PC区、PE区、TA区、TE区和OA区），仅在胚胎期和新生期检测到少量水平细胞。在成年期，生长抑素细胞的数量比早期阶段（E140和新生期）少得多。与其他皮质区域相比，枕叶皮质（OC区）在发育过程中细胞数量变化不大。在枕颞叶皮质，所有阶段从后部到前部细胞数量均增加。早期大脑皮质各层中大量的生长抑素细胞表明，生长抑素在猕猴大脑皮质的发育中起作用。
