Plischuk Santiago, Sanscrainte Neil D, Becnel James J, Estep Alden S, Lange Carlos E
Centro de Estudios Parasitológicos y de Vectores - CEPAVE (CCT La Plata CONICET - UNLP), La Plata, Buenos Aires, Argentina.
Center for Medical, Agricultural and Veterinary Entomology - CMAVE (USDA, ARS), Gainesville, FL, USA.
J Invertebr Pathol. 2015 Mar;126:31-42. doi: 10.1016/j.jip.2015.01.006. Epub 2015 Jan 28.
An undescribed microsporidium was detected and isolated from the South American bumble bee Bombus atratus collected in the Pampas region of Argentina. Infection intensity in workers averaged 8.2 × 10(7)spores/bee. The main site of infection was adipose tissue where hypertrophy of adipocytes resulted in cyst-like body formation. Mature spores were ovoid and monomorphic. They measured 4.00 μm × 2.37 μm (fresh) or 3.98 μm × 1.88 μm (fixed). All stages were diplokariotic and developed in direct contact with host cytoplasm. Isofilar polar filament was arranged in 16 coils in one or, posteriorly, two layers. Coiling angle was variable, between perpendicular and almost parallel to major spore axis. Late meronts and sporogonial stages were surrounded by vesicles of approximately 60 nm in diameter. Based on both new and already designed primers, a 1827 bp (SSUrRNA, ITS, LSUrRNA) sequence was obtained. Data analyses suggest that this microsporidium is a new species of the genus Tubulinosema. The name Tubulinosema pampeana sp. n. is proposed.
从阿根廷潘帕斯地区采集的南美熊蜂（Bombus atratus）中检测并分离出一种未描述过的微孢子虫。工蜂的感染强度平均为8.2×10⁷个孢子/只蜜蜂。主要感染部位是脂肪组织，脂肪细胞肥大导致形成囊状体。成熟孢子呈卵圆形且形态单一。新鲜孢子大小为4.00μm×2.37μm，固定后为3.98μm×1.88μm。所有阶段均为双核，且在与宿主细胞质直接接触的情况下发育。等丝极丝在一层或后部两层中呈16圈排列。盘绕角度可变，介于垂直于和几乎平行于孢子主轴之间。晚期裂殖体和孢子生殖阶段被直径约60nm的囊泡包围。基于新设计的引物和已有的引物，获得了一条1827bp（小亚基核糖体RNA、内转录间隔区、大亚基核糖体RNA）序列。数据分析表明，这种微孢子虫是Tubulinosema属的一个新物种。提出了Tubulinosema pampeana sp. n.这个名称。
