Alvarado Helen L, Abrego Guadalupe, Garduño-Ramirez María L, Clares Beatriz, García María L, Calpena Ana C
Department of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of Barcelona, Joan XXIII Av. s/n, Barcelona 08028, Spain; Department of Physical Chemistry, Faculty of Pharmacy, University of Barcelona, Joan XXIII Av. s/n, Barcelona 08028, Spain.
Centro de Investigaciones Químicas, Universidad Autónoma del Estado de Morelos, Av. Universidad No. 1001, Col Chamilpa, Cuernavaca, Morelos, Mexico.
J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Mar 1;983-984:111-6. doi: 10.1016/j.jchromb.2015.01.009. Epub 2015 Jan 15.
Oleanolic acid (OA) and ursolic acid (UA) are ubiquitous pentacyclic triterpenes compounds in plants. These triterpenoids exhibit unique, important biological and pharmacological activities. For the investigation and development of topical drug delivery systems of triterpenoids in Plumeria obtusa is essential an adequate detection and quantification method for its application in skin permeation studies. The aim of this study was to develop a HPLC method for the determination of OA/UA from leaves of P. obtusa. Results showed that it was sensitive, repeatable, selective, accurate and precise. The detection limit was 0.93±0.21μg/mL and the quantification limit 2.81±0.65μg/mL. Determination coefficients were higher than 0.999 for concentrations between 3.62 and 116μg/mL. The intra and inter-day precision (relative standard deviation) was less than 1.50% and accuracy in terms of relative error ranged between -1.45 and 1.39%. The proposed HPLC method presented advantageous performance characteristics and it can be considered suitable for the evaluation of OA/UA in ex vivo permeation studies.
齐墩果酸（OA）和熊果酸（UA）是植物中普遍存在的五环三萜类化合物。这些三萜类化合物具有独特且重要的生物学和药理活性。对于鸡蛋花中三萜类化合物局部给药系统的研究和开发而言，在皮肤渗透研究中应用的一种适当的检测和定量方法至关重要。本研究的目的是开发一种用于测定鸡蛋花叶片中OA/UA的高效液相色谱法。结果表明，该方法灵敏、可重复、具有选择性、准确且精密。检测限为0.93±0.21μg/mL，定量限为2.81±0.65μg/mL。浓度在3.62至116μg/mL之间时，测定系数高于0.999。日内和日间精密度（相对标准偏差）小于1.50%，相对误差方面的准确度在-1.45至1.39%之间。所提出的高效液相色谱法具有有利的性能特征，可认为适用于离体渗透研究中OA/UA的评估。
