Department of Pharmacy and Pharmaceutical Technology and Physical Chemistry, Faculty of Pharmacy and Food Science, University of Barcelona, Joan XXIII Av. 29-31, 08028 Barcelona, Spain.
Facultad de Ciencias Química e Ingeniería, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Cuernavaca 62209, Morelos, Mexico.
Biomolecules. 2020 Jun 10;10(6):889. doi: 10.3390/biom10060889.
Prenylated flavanones are polyphenols that have diverse biological properties. The present paper focuses on a HPLC method validation for the quantification of prenylated flavanones (2)-5,7-dihydroxy-6-(3-methyl-2-buten-1-yl)-2-phenyl-2,3-dihydro-4H-1Benzopyran-4-one and derivatives (2)-5,7-bis(acetyloxy)-6-(3-methyl-2-buten-1-yl)-2-phenyl-2,3-dihydro-4H-1-Benzopyran-4-one ; (2)-5-hydroxy-7-methoxy-6-(3-methyl-2-buten-1-yl)-2-phenyl-2,3-dihydro-4H-1-Benzopyran-4-one ; (8)-5-hydroxy-2,2-dimethyl-8-phenyl-3,4,7,8-tetrahydro-2H,6H-Benzo[1,2-b:5,4-b']dipyran-6-one ; and (8)-5-hydroxy-2,2-dimethyl-8-phenyl-7,8-dihydro-2H,6H-Benzo[1,2-b:5,4-b']dipyran-6-one applied in biopharmaceutic studies. The linear relationships are proven with significant correlation coefficients (R ˃ 0.999) in the range of 1.56 to 200 μg/mL with low limits of detection and quantification, on average of 0.4 μg/mL and 1.2 μg/mL, respectively. The validation method used in this work is highly accurate and precise, with values lower than 15%. The relative standard deviation values of repeatability of the instrumental system are demonstrated with less than 0.6% for all studied flavanones. Therefore, the applicability method of the quantification of the prenylated flavanones was established using the permeation of human skin in the Franz cell system. During the method previously described, there was no interference observed from human skin components in ex vivo permeation studies.
烯丙基黄酮是具有多种生物特性的多酚类化合物。本文重点介绍了一种用于定量测定烯丙基黄酮（2）-5,7-二羟基-6-（3-甲基-2-丁烯-1-基）-2-苯基-2,3-二氢-4H-1-苯并吡喃-4-酮和衍生物（2）-5,7-双（乙酰氧基）-6-（3-甲基-2-丁烯-1-基）-2-苯基-2,3-二氢-4H-1-苯并吡喃-4-酮；（2）-5-羟基-7-甲氧基-6-（3-甲基-2-丁烯-1-基）-2-苯基-2,3-二氢-4H-1-苯并吡喃-4-酮；（8）-5-羟基-2,2-二甲基-8-苯基-3,4,7,8-四氢-2H,6H-苯并[1,2-b:5,4-b']二吡喃-6-酮；和（8）-5-羟基-2,2-二甲基-8-苯基-7,8-二氢-2H,6H-苯并[1,2-b:5,4-b']二吡喃-6-酮，应用于生物制药研究。线性关系通过在 1.56 至 200 μg/mL 的范围内证明了显著的相关系数（R ˃ 0.999），具有较低的检测和定量下限，平均分别为 0.4 μg/mL 和 1.2 μg/mL。该工作中使用的验证方法非常准确和精确，其值低于 15%。仪器系统重复性的相对标准偏差值表明，所有研究的黄酮类化合物的相对标准偏差值均小于 0.6%。因此，建立了一种使用 Franz 细胞系统透过人皮肤的方法来定量测定烯丙基黄酮。在之前描述的方法中，在离体渗透研究中没有观察到来自人皮肤成分的干扰。
