Alvarado Helen L, Calpena Ana C, Garduño-Ramírez María L, Ortiz Raúl, Melguizo Consolación, Prados José C, Clares Beatriz
Department of Pharmacy and Pharmaceutical Technology and Physical Chemistry, Faculty of Pharmacy and Food Sciences, University of Barcelona, Barcelona, Spain.
Nanoscience and Nanotechnology Institute (IN2UB), University of Barcelona, Barcelona, Spain.
Anticancer Agents Med Chem. 2018;18(6):847-853. doi: 10.2174/1871520618666180111151846.
Triterpenoids are an important class of natural bioactive products present in many medicinal plants.
The aim of present study is to investigate the antioxidant and anticarcinogenic potential of Oleanolic Acid (OA) and Ursolic Acid (UA) on B16 murine melanoma cell line isolated from , free and loaded in a nanoemulsion (NEm) system.
The nanoemulsion was characterized by dynamic light scattering, transmission electron microscopy. The viscosity was also evaluated. The antioxidant activity was determined by the reduction of 2,2-diphenyl-2- picrylhydrazyl (DPPH) free radical. proliferation studies were determined using the sulforhodamine-B method.
OA/UA natural mixture exhibited high percentage of inhibition of DPPH (86.06% and 85.12%, with and without irradiation). Percentages of inhibition higher than 85% in samples with and without ultraviolet irradiation were recorded when loaded in the NEm system. The natural mixture incorporated into the NEm showed cytotoxic activity from 2.9 µM, whereas the free compounds from 17.4 µM.
We conclude that these pentacyclic triterpenes loaded in a NEm system could be considered as a new potential tool for further investigation as anticancer agents.
三萜类化合物是许多药用植物中存在的一类重要的天然生物活性产物。
本研究旨在研究齐墩果酸（OA）和熊果酸（UA）对分离自……的B16小鼠黑色素瘤细胞系的抗氧化和抗癌潜力，以及其游离形式和负载于纳米乳剂（NEm）体系中的情况。
通过动态光散射、透射电子显微镜对纳米乳剂进行表征。还评估了其粘度。通过2,2-二苯基-2-苦基肼（DPPH）自由基的还原测定抗氧化活性。使用磺酰罗丹明-B法进行增殖研究。
OA/UA天然混合物对DPPH的抑制率较高（有和无辐照时分别为86.06%和85.12%）。负载于NEm体系中的样品，无论有无紫外线辐照，抑制率均高于85%。掺入NEm的天然混合物在2.9 μM时显示出细胞毒性活性，而游离化合物在17.4 μM时显示出细胞毒性活性。
我们得出结论，负载于NEm体系中的这些五环三萜可被视为作为抗癌剂进行进一步研究的一种新的潜在工具。
