Vishnuvardhan Reddy Sultanpuram, Thirumala Mothe, Sasikala Chintalapati, Venkata Ramana Chintalapati
Microbial Ecology Lab, Department of Biochemistry, Mahatma Gandhi University, Anneparthy, Yellareddygudem (PO), Nalgonda-508254, Telangana, India.
Bacterial Discovery Laboratory, Centre for Environment, Institute of Science and Technology, J. N. T. University, Kukatpally, Hyderabad-500085, Telangana, India.
Int J Syst Evol Microbiol. 2015 Nov;65(11):4270-4275. doi: 10.1099/ijsem.0.000572. Epub 2015 Sep 2.
Two novel Gram-stain-positive, rod-shaped, non-motile, non-endospore-forming bacterial strains, S7T and IB5, were isolated from Khavda, India. Based on 16S rRNA gene sequence analysis they were identified as belonging to the class Bacilli, order Bacillales, family Bacillaceae, and were most closely related to Bacillus qingdaonensis CGMCC 1.6134T (97.3 %, sequence similarity), Bacillus halochares LMG 24571T (96.9 %), Bacillus salarius KCTC 3912T (95.6 %) and Bacillus aidingensis DSM 18341T (95.3 %). However, these strains shared only 88.2 % 16S rRNA gene sequence similarity with Bacillus subtilis subsp. subtilis DSM 10T, indicating that strains S7T and IB5 might not be members of the genus Bacillus. The DNA-DNA relatedness of these strains with B. qingdaonensis CGMCC 1.6134T was 42.9 ± 0.8. The cell-wall peptidoglycan of strains S7T and IB5 contained meso-diaminopimelic acid, while the polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, a phospholipid and three unknown lipids. The predominant isoprenoid quinone was MK-7. anteiso-C15 : 0 was the predominant fatty acid. The results of the phylogenetic, chemotaxonomic and biochemical tests allowed a clear differentiation of strains S7T and IB5, suggesting that they represent a novel member of the family Bacillaceae, for which the name Salibacterium halotolerans gen. nov., sp. nov. is proposed. The type strain of Salibacterium halotolerans is S7T ( = KCTC 33658T = CGMCC 1.15324T). Based on the results of the present study, it is also suggested that B. qingdaonensis and B. halochares should be transferred to this novel genus, as Salibacterium qingdaonense comb. nov. and Salibacterium halochares comb. nov., respectively.
从印度哈夫达分离出两株新型革兰氏阳性、杆状、无运动性、不形成芽孢的细菌菌株S7T和IB5。基于16S rRNA基因序列分析，它们被鉴定为属于芽孢杆菌纲、芽孢杆菌目、芽孢杆菌科，与青岛芽孢杆菌CGMCC 1.6134T（序列相似性为97.3%）、盐生芽孢杆菌LMG 24571T（96.9%）、盐沼芽孢杆菌KCTC 3912T（95.6%）和艾丁湖芽孢杆菌DSM 18341T（95.3%）关系最为密切。然而，这些菌株与枯草芽孢杆菌枯草亚种DSM 10T的16S rRNA基因序列相似性仅为88.2%，这表明菌株S7T和IB5可能不是芽孢杆菌属的成员。这些菌株与青岛芽孢杆菌CGMCC 1.6134T的DNA-DNA相关性为42.9±0.8。菌株S7T和IB5的细胞壁肽聚糖含有内消旋二氨基庚二酸，而极性脂质包括双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、一种磷脂和三种未知脂质。主要的类异戊二烯醌是MK-7。anteiso-C15:0是主要脂肪酸。系统发育、化学分类和生化测试结果使菌株S7T和IB5得以明确区分，表明它们代表芽孢杆菌科的一个新成员，为此提出了新属名嗜盐盐杆菌属，新种名嗜盐嗜盐杆菌。嗜盐嗜盐杆菌的模式菌株是S7T（=KCTC 33658T=CGMCC 1.15324T）。基于本研究结果，还建议将青岛芽孢杆菌和盐生芽孢杆菌分别转移到这个新属，分别为青岛嗜盐盐杆菌新组合和盐生嗜盐盐杆菌新组合。
