Sultanpuram Vishnuvardhan Reddy, Mothe Thirumala, Chintalapati Sasikala, Chintalapati Venkata Ramana
Microbial Ecology Laboratory, Department of Applied Biosciences, Mahatma Gandhi University, Anneparthy, Yellareddygudem (PO), Nalgonda, 508254, India.
Bacterial Discovery Laboratory, Centre for Environment, Institute of Science and Technology, J. N. T. University, Kukatpally, Hyderabad, 500085, India.
Arch Microbiol. 2017 Nov;199(9):1303-1309. doi: 10.1007/s00203-017-1403-0. Epub 2017 Jun 26.
Two novel (14B and 7B) Gram-stain-positive, rod-shaped, motile and endospore-forming bacterial strains were isolated from Lonar soda lake, India. Based on 16S rRNA gene sequence analysis, the strains 14B and 7B were identified as belonging to the class Firmibacteria and were most closely related to Bacillus halodurans LMG 7121 (99.7 and 99.8%, respectively), Bacillus okuhidensis LMG 22468 (99.1 and 99.2%, respectively) and other members in the genus Bacillus (<97.0%). However, the DNA-DNA relatedness studies indicated that the strains 14B and 7B were distantly related to B. halodurans LMG 7121 (49.1 ± 0.6 and 45.7 ± 0.6, respectively) and B. okuhidensis LMG 22468 (40.9 ± 0.9 and 42.1 ± 0.5, respectively). The high 16S rRNA gene sequence similarity (99.9%) and DNA-DNA relatedness (88 ± 9) indicated that strains 14B and 7B were members of a single species. The strains grew optimally at a pH of 9.0-9.5 with 2-5% (w/v) NaCl and temperature of 37 °C. Strains 14B and 7B were catalase positive and oxydase negative. The cell wall of strain 14B contained meso-diaminopimelic acid as the diagnostic diamino acid. Polar lipids include diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), an unknown aminophospholipid (APL1) and three unknown lipids (L1-3). The predominant isoprenoid quinone is MK-7. anteiso-C (30.8%) was the predominant fatty acid, and significant proportions of iso-C (24.9%), iso-C (17.9%) and anteiso-C (12.3%) were also detected in strains 14B and 7B. The DNA G+C content of strains 14B and 7B was 41.6 and 41.3 mol%, respectively. The results of molecular, physiological and biochemical tests allowed a clear differentiation of strains 14B and 7B from all other members of the genus Bacillus, for which the name Bacillus alcaliphilum sp. nov. is proposed. The type strain is 14B (=KCTC 33777 = CGMCC 1.15474).
从印度洛纳尔盐湖分离出两株新型（14B和7B）革兰氏染色阳性、杆状、具运动性且能形成芽孢的细菌菌株。基于16S rRNA基因序列分析，菌株14B和7B被鉴定为属于厚壁菌门，并且与嗜碱芽孢杆菌LMG 7121（分别为99.7%和99.8%）、奥库希登斯芽孢杆菌LMG 22468（分别为99.1%和99.2%）以及芽孢杆菌属的其他成员（<97.0%）关系最为密切。然而，DNA - DNA相关性研究表明，菌株14B和7B与嗜碱芽孢杆菌LMG 7121（分别为49.1±0.6和45.7±0.6）以及奥库希登斯芽孢杆菌LMG 22468（分别为40.9±0.9和42.1±0.5）的亲缘关系较远。16S rRNA基因序列的高度相似性（99.9%）和DNA - DNA相关性（88±9）表明菌株14B和7B是同一物种的成员。这些菌株在pH为9.0 - 9.5、NaCl浓度为2 - 5%（w/v）以及温度为37℃的条件下生长最佳。菌株14B和7B过氧化氢酶阳性，氧化酶阴性。菌株14B的细胞壁含有内消旋二氨基庚二酸作为诊断性二氨基酸。极性脂质包括双磷脂酰甘油（DPG）、磷脂酰甘油（PG）、磷脂酰乙醇胺（PE）、一种未知氨基磷脂（APL1）和三种未知脂质（L1 - 3）。主要的类异戊二烯醌是MK - 7。anteiso - C（30.8%）是主要脂肪酸，在菌株14B和7B中也检测到相当比例的iso - C（24.9%）、iso - C（17.9%）和anteiso - C（12.3%）。菌株14B和7B的DNA G + C含量分别为41.6和41.3 mol%。分子、生理和生化测试结果使得菌株14B和7B能够与芽孢杆菌属的所有其他成员明显区分开来，为此提出了嗜碱芽孢杆菌新种（Bacillus alcaliphilum sp. nov.）的名称。模式菌株是14B（=KCTC 33777 = CGMCC
