基于光致电子转移的磷光量子点/溴化乙锭纳米杂化物用于DNA检测
Phosphorescent quantum dots/ethidium bromide nanohybrids based on photoinduced electron transfer for DNA detection.
作者信息
Bi Lin, Yu Yuan-Hua
机构信息
School of Life and Technology, Changchun University of Science and Technology, Changchun 130022, PR China.
School of Life and Technology, Changchun University of Science and Technology, Changchun 130022, PR China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2015 Apr 5;140:479-83. doi: 10.1016/j.saa.2015.01.014. Epub 2015 Jan 17.
Mercaptopropionic acid-capped Mn-doped ZnS quantum dots/ethidium bromide (EB) nanohybrids were constructed for photoinduced electron transfer (PIET) and then used as a room-temperature phosphorescence (RTP) probe for DNA detection. EB could quench the RTP of Mn-doped ZnS QDs by PIET, thereby forming Mn-doped ZnS QDs/EB nanohybrids and storing RTP. Meanwhile, EB could be inserted into DNA and EB could be competitively desorbed from the surface of Mn-doped ZnS QDs by DNA, thereby releasing the RTP of Mn-doped ZnS QDs. Based on this mechanism, a RTP sensor for DNA detection was developed. Under optimal conditions, the detection limit for DNA was 0.045 mg L(-1), the relative standard deviation was 1.7%, and the method linear ranged from 0.2 to 20 mg L(-1). The proposed method was applied to biological fluids, in which satisfactory results were obtained.
构建了巯基丙酸包覆的锰掺杂硫化锌量子点/溴化乙锭（EB）纳米杂化物用于光致电子转移（PIET），然后用作室温磷光（RTP）探针用于DNA检测。EB可通过PIET淬灭锰掺杂硫化锌量子点的RTP，从而形成锰掺杂硫化锌量子点/EB纳米杂化物并储存RTP。同时，EB可插入DNA中，并且DNA可使EB从锰掺杂硫化锌量子点表面竞争性解吸，从而释放锰掺杂硫化锌量子点的RTP。基于此机制，开发了一种用于DNA检测的RTP传感器。在最佳条件下，DNA的检测限为0.045 mg L(-1)，相对标准偏差为1.7%，方法线性范围为0.2至20 mg L(-1)。该方法应用于生物流体，获得了满意的结果。
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