Shanxi Normal University, Linfen 041004, PR China.
Shanxi Normal University, Linfen 041004, PR China.
Biosens Bioelectron. 2014 Sep 15;59:300-6. doi: 10.1016/j.bios.2014.03.076. Epub 2014 Apr 12.
MPA-capped Mn-doped ZnS QDs/DXR nanohybrids (MPA: 3-mercaptopropionic acid; QDs: quantum dots; DXR: cetyltrimethyl ammonium bromide) were constructed via photoinduced electron transfer (PIET) and then used as a room-temperature phosphorescence (RTP) probe for detection of DNA. DXR as a quencher will quench the RTP of Mn-doped ZnS QDs via PIET, thereby forming Mn-doped ZnS QDs/DXR nanohybrids and storing RTP. With the addition of DNA, it will be inserted into DXR and thus DXR will be competitively desorbed from the surface of Mn-doped ZnS QDs, thereby releasing the RTP of Mn-doped ZnS QDs. Based on this, a new method for DNA detection was built. The sensor for DNA has a detection limit of 0.039 mg L(-1) and a linear range from 0.1 to 14 mg L(-1). The present QDs-based RTP method does not need deoxidants or other inducers as required by conventional RTP detection methods, and avoids interference from autofluorescence and the scattering light of the matrix that are encountered in spectrofluorometry. Therefore, this method can be used to detect the DNA content in body fluid.
MPA（巯基丙酸）封端的 Mn 掺杂 ZnS QD（量子点）/DXR（十六烷基三甲基溴化铵）纳米杂化材料通过光诱导电子转移（PIET）构建，然后用作室温磷光（RTP）探针用于检测 DNA。DXR 作为猝灭剂将通过 PIET 猝灭 Mn 掺杂 ZnS QD 的 RTP，从而形成 Mn 掺杂 ZnS QD/DXR 纳米杂化材料并存储 RTP。随着 DNA 的加入，它将插入到 DXR 中，从而使 DXR 从 Mn 掺杂 ZnS QD 的表面上竞争解吸，从而释放 Mn 掺杂 ZnS QD 的 RTP。基于此，建立了一种新的 DNA 检测方法。该 DNA 传感器的检测限为 0.039 mg L(-1)，线性范围为 0.1 至 14 mg L(-1)。与传统的 RTP 检测方法所需的氧化剂或其他诱导剂不同，本基于 QD 的 RTP 方法避免了在荧光光谱法中遇到的自发荧光和基质散射光的干扰。因此，该方法可用于检测体液中的 DNA 含量。
