Iguchi H, Hara N, Miyazaki K, Ohtsu Y, Sonoda F, Ohta M
Department of Biochemistry, National Kyushu Cancer Center, Fukuoka, Japan.
Cancer. 1989 Apr 1;63(7):1327-30. doi: 10.1002/1097-0142(19890401)63:7<1327::aid-cncr2820630717>3.0.co;2-q.
Sialyl stage-specific mouse embryonic antigen (SSEA-1) levels were measured in pleural effusions obtained from patients with lung cancer and benign pulmonary disease, using a solid-phase immunoradiometric sandwich assay. The mean (+/- SEM) levels (unit/ml) of pleural fluid sialyl SSEA-1 were 3620 +/- 1419 in adenocarcinoma (n = 25), 123 +/- 30 in nonadenocarcinoma (n = 13) and 95 +/- 19 in benign pulmonary disease (n = 13), respectively. The positive rate was 64% in adenocarcinoma, 7.7% in nonadenocarcinoma, and 0% in benign pulmonary disease, respectively, when a cutoff level was defined as the mean + 3 SD value (300 unit/ml) based on pleural fluid sialyl SSEA-1 levels in benign pulmonary disease. There was a significant positive correlation between pleural fluid levels of sialyl SSEA-1 and those of carcinoembryonic antigen in adenocarcinoma patients (r = 0.8246, P less than 0.01). Pleural fluid sialyl SSEA-1 levels correlated with cytologic findings in adenocarcinoma patients. These observations suggest that sialyl SSEA-1 in pleural effusion is a useful marker to discriminate malignant from nonmalignant and adenocarcinoma from nonadenocarcinoma of the lung.
采用固相免疫放射分析夹心测定法，检测了肺癌患者和良性肺部疾病患者胸腔积液中唾液酸化阶段特异性小鼠胚胎抗原（SSEA-1）的水平。腺癌患者（n = 25）胸腔积液唾液酸化SSEA-1的平均（±SEM）水平（单位/毫升）为3620±1419，非腺癌患者（n = 13）为123±30，良性肺部疾病患者（n = 13）为95±19。当以基于良性肺部疾病患者胸腔积液唾液酸化SSEA-1水平的均值 + 3标准差（300单位/毫升）为临界值时，腺癌的阳性率为64%，非腺癌为7.7%，良性肺部疾病为0%。腺癌患者胸腔积液中唾液酸化SSEA-1水平与癌胚抗原水平之间存在显著正相关（r = 0.8246，P < 0.01）。腺癌患者胸腔积液唾液酸化SSEA-1水平与细胞学检查结果相关。这些观察结果表明，胸腔积液中的唾液酸化SSEA-1是区分肺恶性与非恶性以及腺癌与非腺癌的有用标志物。
