微小RNA-429通过靶向锌指E盒结合蛋白1抑制口腔鳞状细胞癌生长。
MiR-429 inhibits oral squamous cell carcinoma growth by targeting ZEB1.
作者信息
Lei Wanke, Liu Yun-e, Zheng Yuzhu, Qu Lin
机构信息
Department of Stomatology, People's Hospital of Mianzhu, Mianzhu, Sichuan, China (mainland).
New Era Stroke Care and Research Institute, The PLA Second Artillery General Hospital, Beijing, China (mainland).
出版信息
Med Sci Monit. 2015 Feb 2;21:383-9. doi: 10.12659/MSM.893412.
BACKGROUND
Oral squamous cell carcinoma (OSCC) is the sixth most common human malignancy worldwide. To develop new therapeutics requires elucidation of the underlying mechanism of OSCC pathogenesis. The role of miR-429 in OSCC remains unknown.
MATERIAL/METHODS: The level of miR-429 and ZEB1 in OSCC tissues and cell lines was measured by qRT-PCR. MiR-429 was down-regulated by miRNAs antisense oligonucleotides (ASO) transfection and up-regulated by miRNAs mimics. Cell proliferation was analyzed by MTT assay. Cell apoptosis was revealed by FACS analysis. Targeted genes were predicted by a bioinformatics algorithm and confirmed by a dual luciferase reporter assay.
RESULTS
MiR-429 was down-regulated in OSCC tissues, and miR-429 overexpression inhibited OSCC cell lines growth and vice versa. Further, we found that miR-429 could inhibit zinc finger E-boxbinding homeobox 1 (ZEB1) expression, and that miR-429 and ZEB1 expression in OSCC tissues were negatively correlated.
CONCLUSIONS
Our data demonstrate the tumor suppressor role of miR-429 in OSCC, and may provide a potential therapeutic target that warrants further investigation.
背景
口腔鳞状细胞癌（OSCC）是全球第六大常见人类恶性肿瘤。开发新的治疗方法需要阐明OSCC发病机制的潜在机制。miR-429在OSCC中的作用尚不清楚。
材料/方法：采用qRT-PCR检测OSCC组织和细胞系中miR-429和ZEB1的水平。通过miRNAs反义寡核苷酸（ASO）转染下调miR-429，通过miRNAs模拟物上调miR-429。采用MTT法分析细胞增殖。通过FACS分析揭示细胞凋亡。通过生物信息学算法预测靶基因，并通过双荧光素酶报告基因检测进行确认。
结果
miR-429在OSCC组织中表达下调，miR-429过表达抑制OSCC细胞系生长，反之亦然。此外，我们发现miR-429可以抑制锌指E盒结合同源框1（ZEB1）的表达，且OSCC组织中miR-429和ZEB1的表达呈负相关。
结论
我们的数据证明了miR-429在OSCC中的肿瘤抑制作用，并可能提供一个值得进一步研究的潜在治疗靶点。
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