Kearney J N, Franklin U C, Aguirregoicoa V, Holland K T
Yorkshire Regional Tissue Bank, Pinderfields General Hospital, Wakefield, UK.
J Hosp Infect. 1989 Jan;13(1):71-80. doi: 10.1016/0195-6701(89)90097-2.
The ability of ethylene oxide (ETO) gas to penetrate tissue matrices was evaluated using Bacillus subtilis var niger (NCTC 10073) spore strips sandwiched between sheets of various tissues exposed to ETO for varying periods. The decimal reduction times (min) obtained using commercially prepared spores were: 7.97 for naked spores, 7.34 between amnion, and 6.39 between amnio-chorion, indicating complete penetration of the matrix by the gas. Similar conclusions were reached using skin and dura mater. The possible protective effect of serum was evaluated by drying spores suspended in serum in different ways, followed by exposure to the gas. Decimal reduction times obtained were: 8.67 (dried at 37 degrees C); 8.09 (dried at 55 degrees C) and 8.97 (freeze-dried) confirming slight protection. However, in all cases sterilization was achieved following 100 min ETO exposure, giving a 2.5 times safety factor for the commercial ethylene oxide sterilizer.
使用夹在暴露于环氧乙烷不同时间段的各种组织片之间的枯草芽孢杆菌黑色变种（NCTC 10073）孢子条，评估环氧乙烷（ETO）气体穿透组织基质的能力。使用商业制备的孢子获得的十进制减少时间（分钟）为：裸孢子7.97，羊膜之间7.34，羊膜 - 绒毛膜之间6.39，表明气体完全穿透了基质。使用皮肤和硬脑膜也得出了类似的结论。通过以不同方式干燥悬浮在血清中的孢子，然后暴露于气体，评估血清可能的保护作用。获得的十进制减少时间为：8.67（在37摄氏度下干燥）；8.09（在55摄氏度下干燥）和8.97（冷冻干燥），证实有轻微保护作用。然而，在所有情况下，暴露于环氧乙烷100分钟后均实现了灭菌，为商业环氧乙烷灭菌器提供了2.5倍的安全系数。
