Braybrook Siobhan A
The Sainsbury Laboratory, University of Cambridge, Cambridge, UK.
Methods Cell Biol. 2015;125:237-54. doi: 10.1016/bs.mcb.2014.10.006. Epub 2015 Jan 8.
The physical properties of biological materials impact their functions. This is most evident in plants where the cell wall contains each cell's contents and connects each cell to its neighbors irreversibly. Examining the physical properties of the plant cell wall is key to understanding how plant cells, tissues, and organs grow and gain the shapes important for their respective functions. Here, we present an atomic force microscopy-based nanoindentation method for examining the elasticity of plant cells at the subcellular, cellular, and tissue level. We describe the important areas of experimental design to be considered when planning and executing these types of experiments and provide example data as illustration.
生物材料的物理特性会影响其功能。这在植物中最为明显，植物的细胞壁包裹着每个细胞的内容物，并将每个细胞与其相邻细胞不可逆地连接起来。研究植物细胞壁的物理特性是理解植物细胞、组织和器官如何生长以及获得对其各自功能至关重要的形状的关键。在此，我们提出一种基于原子力显微镜的纳米压痕方法，用于在亚细胞、细胞和组织水平上检测植物细胞的弹性。我们描述了在规划和执行这类实验时需要考虑的实验设计的重要方面，并提供示例数据作为说明。
