Chae Young Kee, Kim Seol Hyun, Ellinger James E, Markley John L
Department of Chemistry, Sejong University, Seoul 143-747, Korea.
Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA.
Bull Korean Chem Soc. 2013 Dec;34(12):3602-3608. doi: 10.5012/bkcs.2013.34.12.3602.
, which is a common species of yeast, is by far the most extensively studied model of a eukaryote because although it is one of the simplest eukaryotes, its basic cellular processes resemble those of higher organisms. In addition, yeast is a commercially valuable organism for ethanol production. Since the yeast data can be extrapolated to the important aspects of higher organisms, many researchers have studied yeast metabolism under various conditions. In this report, we analyzed and compared metabolites of under salt and pH stresses of various strengths by using two-dimensional NMR spectroscopy. A total of 31 metabolites were identified for most of the samples. The levels of many identified metabolites showed gradual or drastic increases or decreases depending on the severity of the stresses involved. The statistical analysis produced a holistic outline: pH stresses were clustered together, but salt stresses were spread out depending on the severity. This work could provide a link between the metabolite profiles and mRNA or protein profiles under representative and well studied stress conditions.
酿酒酵母是一种常见的酵母物种，是目前研究最广泛的真核生物模型，因为尽管它是最简单的真核生物之一，但其基本细胞过程与高等生物相似。此外，酵母是用于乙醇生产的具有商业价值的生物体。由于酵母数据可以外推到高等生物的重要方面，许多研究人员在各种条件下研究了酵母代谢。在本报告中，我们使用二维核磁共振光谱分析并比较了酿酒酵母在不同强度的盐和pH胁迫下的代谢物。大多数样品共鉴定出31种代谢物。许多已鉴定代谢物的水平根据所涉及胁迫的严重程度呈现出逐渐或急剧的增加或减少。统计分析得出了一个整体概况：pH胁迫聚集在一起，但盐胁迫根据严重程度分散开来。这项工作可以在具有代表性且研究充分的胁迫条件下，建立代谢物谱与mRNA或蛋白质谱之间的联系。
