谷胱甘肽合成能力增强可提高大肠杆菌对辐射的抗性：一种可能的哺乳动物细胞保护模型。
Increased capacity for glutathione synthesis enhances resistance to radiation in Escherichia coli: a possible model for mammalian cell protection.
作者信息
Moore W R, Anderson M E, Meister A, Murata K, Kimura A
机构信息
Department of Biochemistry, Cornell University Medical College, New York, NY 10021.
出版信息
Proc Natl Acad Sci U S A. 1989 Mar;86(5):1461-4. doi: 10.1073/pnas.86.5.1461.
Abstract
A strain of Escherichia coli, enriched in its content of gamma-glutamylcysteine synthetase and glutathione synthetase activities by recombinant DNA techniques, is more resistant to the lethal effects of gamma-irradiation than is the corresponding wild strain. Although the gene-enriched strain has higher glutathione levels than the wild strain, the observed radioresistance appears to be associated with the increased capacity of the gene-enriched strain to synthesize glutathione when irradiated rather than to the cellular levels of glutathione per se. Thus, resistance was abolished in the presence of buthionine sulfoximine, a selective inactivator of gamma-glutamylcysteine synthetase that decreases glutathione synthesis but that does not act directly to lower cellular glutathione levels. Conclusions drawn from studies on this E. coli model system may have relevance to protection of mammalian cells by glutathione.
摘要
通过重组DNA技术使其γ-谷氨酰半胱氨酸合成酶和谷胱甘肽合成酶活性含量增加的一株大肠杆菌，比相应的野生菌株对γ辐射的致死效应更具抗性。尽管基因富集菌株的谷胱甘肽水平高于野生菌株，但观察到的抗辐射性似乎与基因富集菌株在受到辐射时合成谷胱甘肽的能力增强有关，而不是与细胞内谷胱甘肽本身的水平有关。因此，在丁硫氨酸亚砜胺存在的情况下抗性消失，丁硫氨酸亚砜胺是γ-谷氨酰半胱氨酸合成酶的选择性灭活剂，可降低谷胱甘肽合成，但不直接作用于降低细胞内谷胱甘肽水平。从对这个大肠杆菌模型系统的研究中得出的结论可能与谷胱甘肽对哺乳动物细胞的保护作用有关。
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