Jones Jeff R, Tackenberg Michael C, McMahon Douglas G
Neuroscience Graduate Program, Vanderbilt University, Nashville, Tennessee, USA.
1] Neuroscience Graduate Program, Vanderbilt University, Nashville, Tennessee, USA. [2] Department of Biological Sciences, Vanderbilt University, Nashville, Tennessee, USA.
Nat Neurosci. 2015 Mar;18(3):373-5. doi: 10.1038/nn.3937. Epub 2015 Feb 2.
To examine the interaction between molecular, electrical and behavioral circadian rhythms, we combined optogenetic manipulation of suprachiasmatic nucleus (SCN) firing rate with bioluminescence imaging and locomotor activity monitoring. Manipulating firing rate reset circadian rhythms both ex vivo and in vivo, and this resetting required spikes and network communication. This suggests that SCN firing rate is fundamental to circadian pacemaking as both an input to and output of the molecular clockworks.
为了研究分子、电和行为昼夜节律之间的相互作用，我们将视交叉上核（SCN）放电率的光遗传学操纵与生物发光成像和运动活动监测相结合。操纵放电率在体外和体内均能重置昼夜节律，而这种重置需要动作电位和网络通讯。这表明SCN放电率作为分子生物钟机制的输入和输出，对于昼夜节律起搏至关重要。
