Kim Heung Chul, Takhampunya Ratree, Tippayachai Bousaraporn, Chong Sung-Tae, Park Jee-Yong, Kim Myung-Soon, Seo Hyun-Ji, Yeh Jung-Yong, Lee Won-Ja, Lee Dong-Kyu, Klein Terry A
5th Medical Detachment, 168th Multifunctional Medical Battalion, 65th Medical Brigade, Unit 15247, APO AP 96205-5247, Yongsan, Seoul, Republic of Korea.
Entomology Department, Armed Forces Research Institute of Medical Sciences, 315/6 Rajvithi Road, Bangkok 10400, Thailand.
Mil Med. 2015 Feb;180(2):158-67. doi: 10.7205/MILMED-D-14-00206.
A total of 150,805 culicine female mosquitoes were captured by Mosquito Magnet, black light, and New Jersey light traps, and at resting collections in the Republic of Korea from 2008 to 2010 as part of the U.S. Forces Korea malaria and Japanese surveillance programs. Mosquitoes were identified and culicine mosquitoes placed in pools of up to 30 mosquitoes each, by species and date of collection, and screened for flaviviruses using a reverse transcription-polymerase chain reaction assay. A total of 98/6,845 (1.4%) pools were positive by reverse transcription-polymerase chain reaction for Japanese encephalitis virus (JEV). A total of 92/2,031 (4.5%) pools of Culex tritaeniorhynchus were positive for JEV and accounted for 93.9% (92/98) of all JEV positive pools. A total of 4/804 (0.5%) and 2/175 (1.1%) pools of C. pipiens and C. bitaeniorhynchus, respectively, were positive for JEV. The JEV maximum likelihood estimations (estimated number of viral RNA positive mosquitoes per 1,000) for C. tritaeniorhynchus, C. bitaeniorhynchus, and C. pipiens were 1.71, 1.02, and 0.36 respectively. JEV is a severe health threat for local populations and of significant concern for nonimmune (unvaccinated) U.S. soldiers, civilians, and family members deployed to the Republic of Korea.
作为驻韩美军疟疾和日本脑炎监测项目的一部分，2008年至2010年期间，利用诱蚊器、黑光灯和新泽西诱蚊灯诱捕器，并在韩国进行静息采集，共捕获150,805只库蚊属雌蚊。按蚊种和采集日期将蚊子分类，将库蚊属蚊子每30只分为一组，采用逆转录-聚合酶链反应检测法对黄病毒进行筛查。共有98/6,845（1.4%）组样本经逆转录-聚合酶链反应检测呈日本脑炎病毒（JEV）阳性。三带喙库蚊共有92/2,031（4.5%）组样本JEV呈阳性，占所有JEV阳性样本的93.9%（92/98）。尖音库蚊指名亚种和二带喙库蚊分别有4/804（0.5%）和2/175（1.1%）组样本JEV呈阳性。三带喙库蚊、二带喙库蚊和尖音库蚊指名亚种的JEV最大似然估计值（每1000只病毒RNA阳性蚊子的估计数量）分别为1.71、1.02和0.36。JEV对当地居民构成严重健康威胁，对于部署到韩国的未接种疫苗的美国士兵、平民和家属而言，也备受关注。
