大肠杆菌定居因子抗原I基因的分子克隆及核苷酸序列
Molecular cloning and nucleotide sequence of the colonization factor antigen I gene of Escherichia coli.
作者信息
Karjalainen T K, Evans D G, So M, Lee C H
机构信息
Department of Pathology, Indiana University School of Medicine, Indianapolis 46223.
出版信息
Infect Immun. 1989 Apr;57(4):1126-30. doi: 10.1128/iai.57.4.1126-1130.1989.
Abstract
The colonization factor antigen I (CFA/I) gene has been isolated and sequenced. The amino acid sequence of CFA/I deduced from the nucleotide sequence is composed of 170 amino acids. The first 23 amino acids are considered to be the signal peptide of the CFA/I protein since they are not present in the protein sequence. Among the remaining amino acids, only two are different from the protein sequence: amino acid position 76 is an aspartic acid instead of an asparagine, and position 97 is a serine instead of an alanine. The CFA/I gene has a typical Shine-Dalgarno sequence located 10 base pairs (bp) upstream from the initiation codon. The sequence TACAAT located 48 bp upstream from the initiation codon was tentatively designated the -10 sequence of the CFA/I gene promoter. No sequences homologous to the consensus -35 promoter sequence was found. A pair of inverted repeat sequences followed by a stretch of eight A's are located 45 bp downstream from the termination codon of the CFA/I gene; this region may be a rho-independent transcriptional terminator.
摘要
定居因子抗原I（CFA/I）基因已被分离并测序。从核苷酸序列推导的CFA/I氨基酸序列由170个氨基酸组成。前23个氨基酸被认为是CFA/I蛋白的信号肽，因为它们不存在于蛋白质序列中。在其余氨基酸中，只有两个与蛋白质序列不同：氨基酸位置76是天冬氨酸而非天冬酰胺，位置97是丝氨酸而非丙氨酸。CFA/I基因在起始密码子上游10个碱基对（bp）处有一个典型的Shine-Dalgarno序列。在起始密码子上游48 bp处的序列TACAAT被暂定命名为CFA/I基因启动子的-10序列。未发现与共有-35启动子序列同源的序列。一对反向重复序列后接一段8个A，位于CFA/I基因终止密码子下游45 bp处；该区域可能是一个不依赖ρ的转录终止子。
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本文引用的文献
1
Identification of common molecular subsequences.常见分子子序列的鉴定
J Mol Biol. 1981 Mar 25;147(1):195-7. doi: 10.1016/0022-2836(81)90087-5.
2
Detection of enterotoxigenic Escherichia coli colonization factor antigen I in stool specimens by an enzyme-linked immunosorbent assay.通过酶联免疫吸附测定法检测粪便标本中的产肠毒素大肠杆菌定居因子抗原I
J Clin Microbiol. 1980 Dec;12(6):738-43. doi: 10.1128/jcm.12.6.738-743.1980.
3
Two mannose-resistant haemagglutinins on enterotoxigenic Escherichia coli of serotype O6:K15:H16 or H-isolated from travellers' and infantile diarrhoea.从旅行者腹泻和婴幼儿腹泻中分离出的血清型为O6:K15:H16或H的产肠毒素大肠杆菌上的两种抗甘露糖血凝素。
J Gen Microbiol. 1982 Sep;128(9):2081-96. doi: 10.1099/00221287-128-9-2081.
4
A new solid-state reagent to iodinate proteins. I. Conditions for the efficient labeling of antiserum.一种用于蛋白质碘化的新型固态试剂。I. 高效标记抗血清的条件。
Anal Biochem. 1982 Sep 15;125(2):427-32. doi: 10.1016/0003-2697(82)90025-2.
5
Studies on transformation of Escherichia coli with plasmids.大肠杆菌质粒转化的研究。
J Mol Biol. 1983 Jun 5;166(4):557-80. doi: 10.1016/s0022-2836(83)80284-8.
6
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Ann Microbiol (Paris). 1983 Jan-Feb;134A(1):53-64. doi: 10.1016/0769-2609(83)90103-5.
7
New M13 vectors for cloning.用于克隆的新型M13载体。
Methods Enzymol. 1983;101:20-78. doi: 10.1016/0076-6879(83)01005-8.
8
pEMBL: a new family of single stranded plasmids.pEMBL：一类新型单链质粒。
Nucleic Acids Res. 1983 Mar 25;11(6):1645-55. doi: 10.1093/nar/11.6.1645.
9
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J Bacteriol. 1983 Mar;153(3):1352-60. doi: 10.1128/jb.153.3.1352-1360.1983.
10
Mapping of a plasmid, coding for colonization, factor antigen I and heat-stable enterotoxin production, isolated from an enterotoxigenic strain of Escherichia coli.从产肠毒素大肠杆菌菌株中分离出的一种编码定居因子抗原I和热稳定肠毒素的质粒的图谱分析。
J Bacteriol. 1982 Jan;149(1):264-75. doi: 10.1128/jb.149.1.264-275.1982.
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