Welkos S L, Lowe J R, Eden-McCutchan F, Vodkin M, Leppla S H, Schmidt J J
Bacteriology Division, U.S. Army Medical Research Institute of Infectious Diseases, Frederick, MD 21701-5011.
Gene. 1988 Sep 30;69(2):287-300. doi: 10.1016/0378-1119(88)90439-8.
The nucleotide sequence of the protective antigen (PA) gene from Bacillus anthracis and the 5' and 3' flanking sequences were determined. PA is one of three proteins comprising anthrax toxin; and its nucleotide sequence is the first to be reported from B. anthracis. The open reading frame (ORF) is 2319 bp long, of which 2205 bp encode the 735 amino acids of the secreted protein. This region is preceded by 29 codons, which appear to encode a signal peptide having characteristics in common with those of other secreted proteins. A consensus TATAAT sequence was located at the putative -10 promoter site. A Shine-Dalgarno site similar to that found in genes of other Bacillus sp. was located 7 bp upstream from the ATG start codon. The codon usage for the PA gene reflected its high A + T (69%) base composition and differed from those of genes for bacterial proteins from most other sequences examined. The TAA translation stop codon was followed by an inverted repeat forming a potential termination signal. In addition, a 192-codon ORF of unknown significance, theoretically encoding a 21.6-kDa protein, preceded the 5' end of the PA gene.
测定了炭疽芽孢杆菌保护性抗原（PA）基因的核苷酸序列及其5'和3'侧翼序列。PA是构成炭疽毒素的三种蛋白质之一；其核苷酸序列是首次从炭疽芽孢杆菌中报道的。开放阅读框（ORF）长2319 bp，其中2205 bp编码分泌蛋白的735个氨基酸。该区域之前有29个密码子，似乎编码一个具有与其他分泌蛋白共同特征的信号肽。在假定的 -10启动子位点发现了一个共有序列TATAAT。一个与其他芽孢杆菌属基因中发现的类似的Shine-Dalgarno位点位于ATG起始密码子上游7 bp处。PA基因的密码子使用情况反映了其高A + T（69%）的碱基组成，并且与大多数其他检测序列的细菌蛋白质基因不同。TAA翻译终止密码子之后是一个反向重复序列，形成一个潜在的终止信号。此外，在PA基因的5'端之前有一个192个密码子的ORF，其意义未知，理论上编码一种21.6 kDa的蛋白质。
