Bar-Or David, Bar-Or Raphael, Rael Leonard T, Brody Edward N
Swedish Medical Center, Englewood, CO, USA; St. Anthony Hospital, Lakewood, CO, USA; The Medical Center of Plano, Plano, TX, USA.
SomaLogic, Inc., Boulder, CO, USA.
Redox Biol. 2015;4:340-5. doi: 10.1016/j.redox.2015.01.006. Epub 2015 Jan 13.
The overall redox potential of a cell is primarily determined by oxidizable/reducible chemical pairs, including glutathione-glutathione disulfide, reduced thioredoxin-oxidized thioredoxin, and NAD(+)-NADH (and NADP-NADPH). Current methods for evaluating oxidative stress rely on detecting levels of individual byproducts of oxidative damage or by determining the total levels or activity of individual antioxidant enzymes. Oxidation-reduction potential (ORP), on the other hand, is an integrated, comprehensive measure of the balance between total (known and unknown) pro-oxidant and antioxidant components in a biological system. Much emphasis has been placed on the role of oxidative stress in chronic diseases, such as Alzheimer's disease and atherosclerosis. The role of oxidative stress in acute diseases often seen in the emergency room and intensive care unit is considerable. New tools for the rapid, inexpensive measurement of both redox potential and total redox capacity should aid in introducing a new body of literature on the role of oxidative stress in acute illness and how to screen and monitor for potentially beneficial pharmacologic agents.
细胞的整体氧化还原电位主要由可氧化/可还原的化学对决定，包括谷胱甘肽-谷胱甘肽二硫化物、还原型硫氧还蛋白-氧化型硫氧还蛋白，以及NAD(+)-NADH（和NADP-NADPH）。目前评估氧化应激的方法依赖于检测氧化损伤的单个副产物水平，或测定单个抗氧化酶的总水平或活性。另一方面，氧化还原电位（ORP）是生物系统中总（已知和未知）促氧化剂和抗氧化剂成分之间平衡的一种综合、全面的度量。氧化应激在诸如阿尔茨海默病和动脉粥样硬化等慢性疾病中的作用已受到广泛关注。氧化应激在急诊室和重症监护病房常见的急性疾病中的作用也相当大。用于快速、廉价测量氧化还原电位和总氧化还原能力的新工具，应有助于引入关于氧化应激在急性疾病中的作用以及如何筛选和监测潜在有益药物的新文献。
