Smith Geary R, Bauer Liesel, Crane Maria M, Johnson Zachary P
Division of Animal Resources, Yerkes National Primate Research Center, Emory University, Atlanta, GA, USA.
J Med Primatol. 2015 Apr;44(2):76-88. doi: 10.1111/jmp.12161. Epub 2015 Jan 21.
African non-human primates are SIV natural hosts and do not develop disease following infection. Understanding disease avoidance mechanisms in these species is important for HIV vaccine development. The largest captive population of sooty mangabeys, a SIV natural host species, resides at the Yerkes National Primate Research Center.
Thirteen primer sets that amplify polymorphic microsatellite loci within the MHC region were used to genotype 144 animals. Immunogenetic Management Software (IMS) was used to identify MHC haplotypes and organize data.
Seventy-three haplotypes were identified. Limited haplotype diversity was observed in this population with 88.2% of included animals carrying one of 18 haplotypes. Differences in haplotype frequency were observed between SIV (+) and SIV (-) populations.
We have developed a novel tool for others to use in the analysis of the role of the MHC in a natural host non-human primate model species used for SIV research.
非洲非人灵长类动物是猴免疫缺陷病毒（SIV）的天然宿主，感染后不会发病。了解这些物种的疾病规避机制对HIV疫苗研发至关重要。煤烟狒狒是一种SIV天然宿主物种，其最大的圈养种群位于耶基斯国家灵长类动物研究中心。
使用13组引物对MHC区域内的多态微卫星位点进行扩增，对144只动物进行基因分型。使用免疫遗传学管理软件（IMS）识别MHC单倍型并整理数据。
共识别出73种单倍型。该种群观察到有限的单倍型多样性，88.2%的纳入动物携带18种单倍型中的一种。在SIV（+）和SIV（-）种群之间观察到单倍型频率的差异。
我们开发了一种新工具，供其他人用于分析MHC在用于SIV研究的天然宿主非人灵长类动物模型物种中的作用。
