Heimbruch Katelyn E, Karl Julie A, Wiseman Roger W, Dudley Dawn M, Johnson Zach, Kaur Amitinder, O'Connor David H
Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, WI, 53715, USA.
Immunogenetics. 2015 Aug;67(8):437-45. doi: 10.1007/s00251-015-0847-0. Epub 2015 May 26.
Sooty mangabeys (Cercocebus atys) are natural SIV hosts and the presumed source of HIV-2 and SIVmac, which makes them a valuable model for HIV/SIV research. However, like other African primates, little is known about their major histocompatibility complex (MHC) genetics. In this study, we used Roche/454 and Illumina MiSeq deep sequencing in order to determine the MHC class I transcripts in a cohort of 165 sooty mangabeys from the Yerkes National Primate Research Center (YNPRC). We have characterized 121 functionally full-length classical (Ceat-A and Ceat-B) and non-classical (Ceat-F and Ceat-I) alleles and have also identified 22 Ceat-A/Ceat-B haplotype chromosomal combinations. We correlated these Ceat-A/Ceat-B haplotype combinations to recently described microsatellite haplotypes from the YNPRC colony. These newly identified alleles and haplotypes establish a resource for studying cellular immunity in sooty mangabeys and provide a framework for rapidly cataloging MHC class I sequences in an understudied, yet important, nonhuman primate species.
乌白眉猴（Cercocebus atys）是猴免疫缺陷病毒（SIV）的天然宿主，被认为是HIV-2和SIVmac的源头，这使其成为HIV/SIV研究的宝贵模型。然而，与其他非洲灵长类动物一样，人们对它们的主要组织相容性复合体（MHC）遗传学知之甚少。在本研究中，我们使用罗氏/454测序和Illumina MiSeq深度测序，以确定来自耶基斯国家灵长类研究中心（YNPRC）的165只乌白眉猴群体中的MHC I类转录本。我们已鉴定出121个功能上全长的经典（Ceat-A和Ceat-B）和非经典（Ceat-F和Ceat-I）等位基因，还确定了22种Ceat-A/Ceat-B单倍型染色体组合。我们将这些Ceat-A/Ceat-B单倍型组合与YNPRC群体最近描述的微卫星单倍型进行了关联。这些新鉴定的等位基因和单倍型为研究乌白眉猴的细胞免疫建立了资源，并为在一个研究较少但很重要的非人类灵长类物种中快速编目MHC I类序列提供了框架。
