Feldman Mark, Shenderovich Julia, Al-Quntar Abed Al Aziz, Friedman Michael, Steinberg Doron
Biofilm Research Laboratory, Institute of Dental Sciences, Faculty of Dental Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel.
Biofilm Research Laboratory, Institute of Dental Sciences, Faculty of Dental Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel The Institute for Drug Research, School of Pharmacy, The Hebrew University of Jerusalem, Jerusalem, Israel.
Antimicrob Agents Chemother. 2015 Apr;59(4):2265-72. doi: 10.1128/AAC.04212-14. Epub 2015 Feb 2.
Thiazolidinedione-8 (S-8) has recently been identified as a potential anti-quorum-sensing/antibiofilm agent against bacteria and fungi. Based on these results, we investigated the possibility of incorporating S-8 in a sustained-release membrane (SRM) to increase its pharmaceutical potential against Candida albicans biofilm. We demonstrated that SRM containing S-8 inhibits fungal biofilm formation in a time-dependent manner for 72 h, due to prolonged release of S-8. Moreover, the SRM effectively delivered the agent in its active form to locations outside the membrane reservoir. In addition, eradication of mature biofilm by the SRM containing S-8 was also significant. Of note, S-8-containing SRM affected the characteristics of mature C. albicans biofilm, such as thickness, exopolysaccharide (EPS) production, and morphogenesis of fungal cells. The concept of using an antibiofilm agent with no antifungal activity incorporated into a sustained-release delivery system is new in medicine and dentistry. This concept of an SRM containing a quorum-sensing quencher with an antibiofilm effect could pave the way for combating oral fungal infectious diseases.
噻唑烷二酮 -8（S -8）最近被确定为一种针对细菌和真菌的潜在群体感应抑制/抗生物膜剂。基于这些结果，我们研究了将S -8纳入缓释膜（SRM）以提高其抗白色念珠菌生物膜药物潜力的可能性。我们证明，由于S -8的持续释放，含S -8的SRM在72小时内以时间依赖性方式抑制真菌生物膜形成。此外，SRM有效地将活性形式的药物输送到膜储库外的位置。此外，含S -8的SRM对成熟生物膜的根除也很显著。值得注意的是，含S -8的SRM影响成熟白色念珠菌生物膜的特性，如厚度、胞外多糖（EPS）产生和真菌细胞的形态发生。将无抗真菌活性的抗生物膜剂纳入缓释给药系统的概念在医学和牙科领域是新的。这种含有具有抗生物膜作用的群体感应淬灭剂的SRM概念可能为对抗口腔真菌感染性疾病铺平道路。
