Bancone Germana, Chu Cindy S, Chowwiwat Nongnud, Somsakchaicharoen Raweewan, Wilaisrisak Pornpimon, Charunwatthana Prakaykaew, Bansil Pooja, McGray Sarah, Domingo Gonzalo J, Nosten François H
Am J Trop Med Hyg. 2015 Apr;92(4):818-824. doi: 10.4269/ajtmh.14-0696. Epub 2015 Feb 2.
The use of primaquine and other 8-aminoquinolines for malaria elimination is hampered by, among other factors, the limited availability of point-of-care tests for the diagnosis of glucose-6-phosphate dehydrogenase (G6PD) deficiency. Historically, the most used source of blood for G6PD analyses is venous blood, whereas diagnostic devices used in the field require the use of capillary blood; data have shown that the two sources of blood often differ with respect to hemoglobin concentration and number of red blood cells. Therefore, we have analyzed, in both capillary and venous blood drawn from the same healthy donors, the correlation of G6PD activity assessed by two qualitative tests (the Fluorescent Spot test and the CareStart test) with the gold standard quantitative spectrophotometric assay. Results obtained on 150 subjects with normal, intermediate, and deficient G6PD phenotypes show that, although differences exist between the aforementioned characteristics in capillary and venous blood, these do not impact on the quantitative assessment of G6PD activity after corrected for hemoglobin concentration or red blood cell count. Furthermore, we have assessed the sensitivity and specificity of the two qualitative tests against the gold standard spectrophotometric assay at different activity thresholds of residual enzymatic activity in both blood sources.
伯氨喹和其他8-氨基喹啉用于疟疾消除受到多种因素的阻碍，其中包括用于诊断葡萄糖-6-磷酸脱氢酶（G6PD）缺乏症的即时检测方法有限。从历史上看，G6PD分析最常用的血液来源是静脉血，而现场使用的诊断设备需要使用毛细血管血；数据表明，这两种血液来源在血红蛋白浓度和红细胞数量方面往往存在差异。因此，我们在从同一健康供体采集的毛细血管血和静脉血中，分析了两种定性检测方法（荧光斑点试验和CareStart试验）评估的G6PD活性与金标准定量分光光度法之间的相关性。对150名具有正常、中间型和缺陷型G6PD表型的受试者的检测结果表明，尽管毛细血管血和静脉血的上述特征存在差异，但在校正血红蛋白浓度或红细胞计数后，这些差异不会影响G6PD活性的定量评估。此外，我们还评估了在两种血液来源中，针对金标准分光光度法，这两种定性检测方法在不同残余酶活性阈值下的敏感性和特异性。
