Shen N, Qin Y, Wang Q, Liao S, Zhu J, Zhu Q, Mi H, Adhikari B, Wei Y, Huang R
Guangxi Key Laboratory of Subtropical Bio-resource Conservation and Utilization, College of Life Science and Technology, Guangxi University, Nanning, Guangxi, China.
State Key Laboratory of Non-Food Biomass and Enzyme Technology, National Engineering Research Center for Non-Food Biorefinery, Guangxi Academy of Sciences, Nanning, Guangxi, China.
Lett Appl Microbiol. 2015 Jun;60(6):544-51. doi: 10.1111/lam.12399. Epub 2015 Mar 26.
The potential of using corn steep liquor powder (CSLP), peanut meal (PM), soybean meal (SM), cotton meal (CM) and urea as the substitute of yeast extract (YE) as the nitrogen source was investigated for producing succinic acid (SA). Actinobacillus succinogenes GXAS137 was used as the fermenting bacterium and sugarcane molasses was used as the main substrate. None of these materials were able to produce SA as high as YE did. The CSLP could still be considered as a feasible and inexpensive alternate for YE as the yield of SA produced using CSLP was second only to the yield of SA obtained by YE. The use of CSLP-PM mixed formulation (CSLP to PM ratio = 2·6) as nitrogen source produced SA up to 59·2 g l(-1) with a productivity of 1·2 g l(-1) h(-1). A batch fermentation using a stirred bioreactor produced up to 60·7 g l(-1) of SA at the same formulation. Fed-batch fermentation that minimized the substrate inhibition produced 64·7 g l(-1) SA. These results suggest that sugarcane molasses supplemented with a mixture of CSLP and PM as the nitrogen source could be used to produce SA more economically using A. succinogenes. Significance and impact of the study: Succinic acid (SA) is commonly used as a platform chemical to produce a number of high value derivatives. Yeast extract (YE) is used as a nitrogen source to produce SA. The high cost of YE is currently the limiting factor for industrial production of SA. This study reports the use of a mixture of corn steep liquor powder (CSLP) and peanut meal (PM) as an inexpensive nitrogen source to substitute YE. The results showed that this CSLP-PM mixed formulation can be used as an effective and economic nitrogen source for the production of SA.
研究了使用玉米浆干粉（CSLP）、花生粕（PM）、豆粕（SM）、棉粕（CM）和尿素作为酵母提取物（YE）的替代氮源来生产琥珀酸（SA）的潜力。以产琥珀酸放线杆菌GXAS137作为发酵菌株，甘蔗废蜜作为主要底物。这些材料均无法产生与YE一样高产量的SA。CSLP仍可被视为YE可行且廉价的替代品，因为使用CSLP生产的SA产量仅次于YE获得的SA产量。使用CSLP-PM混合配方（CSLP与PM的比例 = 2·6）作为氮源时，SA产量高达59·2 g l(-1) ，生产率为1·2 g l(-1) h(-1)。在相同配方下，使用搅拌式生物反应器进行分批发酵，SA产量高达60·7 g l(-1)。通过补料分批发酵将底物抑制降至最低，可产生64·7 g l(-1) 的SA。这些结果表明，以CSLP和PM的混合物作为氮源补充甘蔗废蜜，可更经济地利用产琥珀酸放线杆菌生产SA。研究的意义和影响：琥珀酸（SA）通常用作平台化学品来生产多种高价值衍生物。酵母提取物（YE）用作生产SA的氮源。YE的高成本目前是SA工业化生产的限制因素。本研究报道了使用玉米浆干粉（CSLP）和花生粕（PM）的混合物作为廉价氮源替代YE。结果表明，这种CSLP-PM混合配方可作为生产SA的有效且经济的氮源。
