State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, People's Republic of China.
Bioresour Technol. 2011 Jan;102(2):1704-8. doi: 10.1016/j.biortech.2010.08.011. Epub 2010 Aug 6.
The enzymatic hydrolysate of spent yeast cells was evaluated as a nitrogen source for succinic acid production by Actinobacillus succinogenes NJ113, using corn fiber hydrolysate as a carbon source. When spent yeast cell hydrolysate was used directly as a nitrogen source, a maximum succinic acid concentration of 35.5 g/l was obtained from a glucose concentration of 50 g/l, with a glucose utilization of 95.2%. Supplementation with individual vitamins showed that biotin was the most likely factor to be limiting for succinic acid production with spent yeast cell hydrolysate. After supplementing spent yeast cell hydrolysate and 90 g/l of glucose with 150 μg/l of biotin, cell growth increased 32.5%, glucose utilization increased 37.6%, and succinic acid concentration was enhanced 49.0%. As a result, when biotin-supplemented spent yeast cell hydrolysate was used with corn fiber hydrolysate, a succinic acid yield of 67.7% was obtained from 70.3 g/l of total sugar concentration, with a productivity of 0.63 g/(l h). Our results suggest that biotin-supplemented spent yeast cell hydrolysate may be an alternative nitrogen source for the efficient production of succinic acid by A. succinogenes NJ113, using renewable resources.
用过的酵母细胞酶解产物被评估为用于产琥珀酸的氮源，所用碳源为玉米纤维水解物，生产菌为 Actinobacillus succinogenes NJ113。当直接使用用过的酵母细胞酶解产物作为氮源时，在葡萄糖浓度为 50 g/L 的条件下，可获得最大 35.5 g/L 的琥珀酸浓度，葡萄糖利用率为 95.2%。补充各种维生素后发现，对于用过的酵母细胞酶解产物而言，生物素最有可能是限制琥珀酸生产的因素。在用 150 μg/L 生物素补充用过的酵母细胞酶解产物和 90 g/L 葡萄糖后，细胞生长增加了 32.5%，葡萄糖利用率增加了 37.6%，琥珀酸浓度提高了 49.0%。因此，当在含有玉米纤维水解物的条件下使用补充了生物素的用过的酵母细胞酶解产物时，从 70.3 g/L 的总糖浓度中获得了 67.7%的琥珀酸产率，生产率为 0.63 g/（L·h）。我们的结果表明，补充了生物素的用过的酵母细胞酶解产物可能是利用可再生资源由 A. succinogenes NJ113 高效生产琥珀酸的替代氮源。
