Omwene Philip Isaac, Yağcıoğlu Meltem, Öcal-Sarihan Zehra Betül, Ertan Fatma, Keris-Sen Ülker Diler, Karagunduz Ahmet, Keskinler Bülent
Department of Environmental Engineering, Gebze Technical University, 41400 Gebze-Kocaeli, Turkey.
Faculty of Agriculture and Environmental Sciences, Muni University, P.O.Box 725, Arua, Uganda.
3 Biotech. 2021 Aug;11(8):389. doi: 10.1007/s13205-021-02939-w. Epub 2021 Jul 29.
Bio-based succinic acid production has attracted global attention since its consideration as a potential replacement to petroleum-based platform chemicals. This study used three different CO sources, namely NaHCO, KCO and MgCO for fermentation of succinic acid (SA) by under three distinct substrate conditions i.e. lactose, whey and whey devoid of any supplements. Batch experiments were performed in both anaerobic flasks and 5L benchtop fermenter. SA fermentation in anaerobic flasks was unfettered by supplementary nutrients. However, fermentation in the benchtop fermenter devoid of supplementary nutrients resulted into 42% reduction in SA yield as well as lower SA productivities. Furthermore, a significant reduction of cell growth occurred in anerobic flasks at pH < 6.0, and complete termination of bacterial activity was noted at pH < 5.3. The highest SA titer, yield and productivity of 15.67 g/L, 0.54 g/g and 0.33 g/L/h, respectively, was recorded from whey fermentation with MgCO. The present study further highlights significant inhibitory effect of KCO buffered medium on . Thus, we can claim that environmental pollution as well as costs of SA production from whey can be reduced by leveraging on whey residual nutrients to support the activity of .
由于生物基琥珀酸被视为石油基平台化学品的潜在替代品，其生产已引起全球关注。本研究使用三种不同的碳源，即碳酸氢钠、碳酸钾和碳酸镁，在三种不同的底物条件下，即乳糖、乳清和无任何补充剂的乳清中，用于[具体微生物名称未给出]发酵生产琥珀酸（SA）。在厌氧瓶和5L台式发酵罐中进行了分批实验。厌氧瓶中的SA发酵不受补充营养物质的影响。然而，在无补充营养物质的台式发酵罐中发酵导致SA产量降低42%，SA生产率也较低。此外，在pH < 6.0时，厌氧瓶中的细胞生长显著减少，在pH < 5.3时，细菌活性完全终止。用碳酸镁进行乳清发酵时，记录到最高的SA滴度、产量和生产率，分别为15.67 g/L、0.54 g/g和0.33 g/L/h。本研究进一步强调了碳酸钾缓冲培养基对[具体微生物名称未给出]的显著抑制作用。因此，我们可以声称，通过利用乳清残留营养物质来支持[具体微生物名称未给出]的活性，可以减少环境污染以及从乳清生产SA的成本。
