Shi Ying, Chen Guo-Bin, Huang Qing-Wen, Chen Xu, Liu Jing-Jing, Xu Wei, Huang Xiao-Xiao, Liu Yun-Peng, Xiao Chuan-Xing, Wu Deng-Chyang, Guleng Bayasi, Ren Jian-Lin
Department of Gastroenterology, Zhongshan Hospital affiliated to Xiamen University, 201 Hubin South Road, Xiamen 361004, Fujian, China.
Department of Gastroenterology, Zhongshan Hospital affiliated to Xiamen University, 201 Hubin South Road, Xiamen 361004, Fujian, China; Department of Gastroenterology, Xiamen Zhongshan Teaching Hospital of Fujian Medical University, Xiamen 361004, Fujian, China.
Biochim Biophys Acta. 2015 May;1852(5):970-9. doi: 10.1016/j.bbadis.2015.01.016. Epub 2015 Jan 31.
Trefoil factor 1 (TFF1), a member of the trefoil peptide family, is not only associated with mucosal protection and restoration but is also correlated with tumorigenesis of the gastrointestinal tract. In an early study, we performed sequence analysis and identified one potential miR423-5p binding site within the 3'-untranslated region of TFF1 using microRNA target prediction tools. In the current study, we demonstrated that the coding DNA region within TFF1 is also a candidate for miR218-5p targeting. We used real-time PCR and in situ hybridization to analyze the correlation between miR218-5p and TFF1 expression in tumor lesions and paracancerous tissue in gastric cancer (GC) samples. Additionally, endogenous and exogenous TFF1 were suppressed by miR218-5p in gastric cancer cells and influenced the progression of GC in an Erk1/2-dependent manner. Targeting miR218-5p may provide a novel strategy for the treatment of GC.
三叶因子1（TFF1）是三叶肽家族的成员之一，不仅与黏膜保护和修复有关，还与胃肠道肿瘤发生相关。在一项早期研究中，我们进行了序列分析，并使用微小RNA靶标预测工具在TFF1的3'-非翻译区内鉴定出一个潜在的miR423-5p结合位点。在当前研究中，我们证明TFF1内的编码DNA区域也是miR218-5p靶向的候选对象。我们使用实时PCR和原位杂交分析胃癌（GC）样本中肿瘤病变和癌旁组织中miR218-5p与TFF1表达之间的相关性。此外，miR218-5p在胃癌细胞中抑制内源性和外源性TFF1，并以依赖Erk1/2的方式影响GC的进展。靶向miR218-5p可能为GC的治疗提供一种新策略。
