Department of Otolaryngology, Head and Neck Surgery, Istanbul Medeniyet University Faculty of Medicine, Istanbul, Turkey.
Dr. Behçet Uz Child Disease and Pediatric Surgery Training and Research Hospital Department of Otolaryngology-Head Neck Surgery, İsmet Kaptan Mh, Sezer Doğan Sok No:11, 35210, Konak/Izmir, Turkey.
J Otolaryngol Head Neck Surg. 2021 Feb 27;50(1):14. doi: 10.1186/s40463-021-00497-y.
The malignancy potential of the laryngeal lesions are one of the major concerns of the surgeons about choosing the treatment options, forming surgical margins, deciding the follow-up periods. Finding a biomarker to overcome these concerns are ongoing challenges and recently microRNAs (miRNAs) are attributed as possible candidates since they can regulate gene expressions in the human genome. The objective of our study was to investigate their capability as a transformation biomarker for malignant laryngeal lesions.
We investigated mature miRNA expressions in paraffin-embedded surgical specimens of human laryngeal tissues grouped as benign, premalignant or malignant (n = 10 in each). miRNA profiling was carried out by quantitative Real-Time polymerase chain reaction (RT-qPCR) and data were analyzed according to fold regulation.
Our results demonstrated that 9 miRNAs were upregulated as the lesions become more malignant. Among them Hs_miR-183_5p, Hs_miR-155_5p, and Hs_miR-106b_3p expressions were significantly 4.16 (p = 0.032), 2.72 (p = 0.028) and 3.01 (p = 0.022) fold upregulated respectively in premalignant lesions compared to the benign lesions. Moreover, their expressions were approximately 2.76 fold higher in the malignant group than in the premalignant group compared to the benign group. Besides them, significant 7.57 (p = 0.036), 4.45 (p = 0.045) and 5.98 (p = 0.023) fold upregulations of Hs_miR-21_5p, Hs_miR-218_3p, and Hs_miR-210_3p were noticed in the malignant group but not in the premalignant group when compared to the benign group, respectively.
MiRNAs might have important value to help the clinicians for their concerns about the malignancy potentials of the laryngeal lesions. Hs_miR-183_5p, Hs_miR-155_5p, and Hs_miR-106b_3p might be followed as transformation marker, whereas Hs_miR-21_5p, Hs_miR-218_3p, and Hs_miR-210_3p might be a biomarker prone to malignancy.
喉部病变的恶性潜能是外科医生选择治疗方案、形成手术边缘、决定随访期的主要关注点之一。寻找一种生物标志物来克服这些担忧是一个持续的挑战，最近 microRNAs (miRNAs) 被认为是可能的候选者，因为它们可以调节人类基因组中的基因表达。我们的研究目的是研究它们作为恶性喉部病变转化生物标志物的能力。
我们调查了石蜡包埋的人类喉部组织手术标本中成熟 miRNA 的表达，这些标本分为良性、癌前病变或恶性（每组 10 例）。通过定量实时聚合酶链反应 (RT-qPCR) 进行 miRNA 谱分析，并根据倍数调节进行数据分析。
我们的结果表明，随着病变变得更恶性，有 9 种 miRNA 上调。其中，Hs_miR-183_5p、Hs_miR-155_5p 和 Hs_miR-106b_3p 的表达分别在癌前病变中比良性病变显著上调 4.16 倍（p=0.032）、2.72 倍（p=0.028）和 3.01 倍（p=0.022）。此外，与良性组相比，恶性组中它们的表达分别约高出 2.76 倍。除此之外，Hs_miR-21_5p、Hs_miR-218_3p 和 Hs_miR-210_3p 的表达也显著上调，分别为 7.57 倍（p=0.036）、4.45 倍（p=0.045）和 5.98 倍（p=0.023），但在癌前病变组中没有上调。
miRNAs 可能具有重要价值，可以帮助临床医生了解喉部病变的恶性潜能。Hs_miR-183_5p、Hs_miR-155_5p 和 Hs_miR-106b_3p 可能作为转化标志物，而 Hs_miR-21_5p、Hs_miR-218_3p 和 Hs_miR-210_3p 可能是恶性倾向的标志物。
